1.7.1.13: preQ1 synthase
This is an abbreviated version!
For detailed information about preQ1 synthase, go to the full flat file.
Reaction
Synonyms
7-aminomethyl-7-carbaguanine:NADP+ oxidoreductase, 7-cyano-7-deazaguanine reductase, NADPH-dependent 7-cyano-7-deazaguanine reductase, nitrile reductase, NRed, preQ0 reductase, preQ0oxidoreductase, QueF, queuine synthase, YkvM
ECTree
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Engineering
Engineering on EC 1.7.1.13 - preQ1 synthase
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C99A
mutant does not compromise enzyme activity. Contrary to wild-type, peroxide-induced inactivation is irreversible
C99S
mutant does not compromise enzyme activity. Contrary to wild-type, peroxide-induced inactivation is irreversible
C99A
-
mutant does not compromise enzyme activity. Contrary to wild-type, peroxide-induced inactivation is irreversible
-
C99S
-
mutant does not compromise enzyme activity. Contrary to wild-type, peroxide-induced inactivation is irreversible
-
C190A
C190S
the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity
D197A
the variant does not take up protons in conjunction with preQ0 binding
D197H
the variant recovers proton uptake to a level almost analogous to that seen with the wildtype enzyme
E230Q
about 4% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one
E89A
the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product
E89L
the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product
F228A
the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product
F228W
about 8% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one
H229A
I192A
the 7-cyano-7-deazaguanine (preQ0) reduction by the mutant gives 7-aminomethyl-7-deazaguanine (preQ1) and 7-formyl-7-deazaguanine at a 1:1 ratio
L191A
the 7-cyano-7-deazaguanine (preQ0) reduction by the mutant gives 7-aminomethyl-7-deazaguanine (preQ1) and 7-formyl-7-deazaguanine at a 4:1 ratio
S90A
about 10% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one
C190A
-
mutation in catalytic residue, no evidence of covalent binding of substrate preQ0. Mutant displays proton uptake
C190A
the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity
about 15% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one
H229A
the variant readily forms the thioimidate adduct and is 24fold less active for preQ0 reduction than wild type enzyme