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1.7.1.13: preQ1 synthase

This is an abbreviated version!
For detailed information about preQ1 synthase, go to the full flat file.

Word Map on EC 1.7.1.13

Reaction

7-aminomethyl-7-carbaguanine
+ 2 NADP+ =
7-cyano-7-carbaguanine
+ 2 NADPH + 2 H+

Synonyms

7-aminomethyl-7-carbaguanine:NADP+ oxidoreductase, 7-cyano-7-deazaguanine reductase, NADPH-dependent 7-cyano-7-deazaguanine reductase, nitrile reductase, NRed, preQ0 reductase, preQ0oxidoreductase, QueF, queuine synthase, YkvM

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.13 preQ1 synthase

Engineering

Engineering on EC 1.7.1.13 - preQ1 synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C99A
mutant does not compromise enzyme activity. Contrary to wild-type, peroxide-induced inactivation is irreversible
C99S
mutant does not compromise enzyme activity. Contrary to wild-type, peroxide-induced inactivation is irreversible
C99A
-
mutant does not compromise enzyme activity. Contrary to wild-type, peroxide-induced inactivation is irreversible
-
C99S
-
mutant does not compromise enzyme activity. Contrary to wild-type, peroxide-induced inactivation is irreversible
-
C190A
C190S
the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity
D197A
the variant does not take up protons in conjunction with preQ0 binding
D197H
the variant recovers proton uptake to a level almost analogous to that seen with the wildtype enzyme
D197N
complete loss of activity
E230Q
about 4% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one
E89A
the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product
E89L
the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product
F228A
the variant performs primarily (more than 90%) a two-electron reduction of 7-cyano-7-deazaguanine (preQ0), releasing hydrolyzed imine (7-formyl-7-deazaguanine) as the product
F228W
about 8% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one
H229A
I192A
the 7-cyano-7-deazaguanine (preQ0) reduction by the mutant gives 7-aminomethyl-7-deazaguanine (preQ1) and 7-formyl-7-deazaguanine at a 1:1 ratio
L191A
the 7-cyano-7-deazaguanine (preQ0) reduction by the mutant gives 7-aminomethyl-7-deazaguanine (preQ1) and 7-formyl-7-deazaguanine at a 4:1 ratio
Q89A
poor activity
Q89L
poor activity
S90A
about 10% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one