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1.5.99.B2: proline dehydrogenase (acceptor)

This is an abbreviated version!
For detailed information about proline dehydrogenase (acceptor), go to the full flat file.

Word Map on EC 1.5.99.B2

Reaction

L-proline
+
a quinone
=
(S)-1-pyrroline-5-carboxylate
+
a quinol

Synonyms

APE_1267.1, dye-linked L-proline dehydrogenase, L-proDH, L-proline dehydrogenase, L-proline: FAD oxidoreductase, L-proline:FAD oxidoreductase, LPDH, PDH, PDH1, PDH2, PF1246, PF1798, PH1364, PH1751, PIG6, POX, Pro dehydrogenase, PRODH, ProDH1, ProDH2, proline dehydrogenase, proline oxidase, prub, PutA, TK0117, TK0122, TPpdhB, TPpdhB2, TtProDH

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.99 With unknown physiological acceptors
                1.5.99.B2 proline dehydrogenase (acceptor)

Specific Activity

Specific Activity on EC 1.5.99.B2 - proline dehydrogenase (acceptor)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.026
-
crude extract, pH 8.0, at 50°C
0.939
-
isoform PDH1
1.4
-
isoform PDH2
28.9
after purification, at 25?C, in 50 mM Tris-HCl (pH 7.5)
3.57
-
pH 6.5, 50°C
4.25
-
after 163fold purification, pH 8.0, at 50°C
600
-
purified recombinant enzyme in an optimized aqueous two-phase system containing 14% w/w PEG-1000 and 12% w/w Na2CO3, pH 8.0, 37°C