1.5.8.4: dimethylglycine dehydrogenase
This is an abbreviated version!
For detailed information about dimethylglycine dehydrogenase, go to the full flat file.
Word Map on EC 1.5.8.4
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1.5.8.4
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sarcosine
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fad
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flavinylated
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folate-binding
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wagner
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medicine
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diagnostics
- 1.5.8.4
- sarcosine
- fad
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flavinylated
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folate-binding
-
wagner
- medicine
- diagnostics
Reaction
Synonyms
Csal_0990, DdhC, DMGDH, EC 1.5.99.2, Me2GlyDH, mMe2GlyDH, N,N-dimethylglycine oxidase, pMe2GlyDH
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Purification
Purification on EC 1.5.8.4 - dimethylglycine dehydrogenase
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native enzyme from liver mitochondria, recombinant His-tagged enzyme from Escherichia coli 45fold by nickel affinity chromatography
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of in vitro synthesized 6-His-dimethylglycine dehydrogenase on nickel chelating Sepharose
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using ammonium sulfate treatment and dissolution in 7.5 mM potassium phosphate
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using gel filtration chromatography on Sephadex G-150, DEAE-cellulose chromatography, and affinity chromatography
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