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1.5.8.4: dimethylglycine dehydrogenase

This is an abbreviated version!
For detailed information about dimethylglycine dehydrogenase, go to the full flat file.

Word Map on EC 1.5.8.4

Reaction

N,N-dimethylglycine
+
5,6,7,8-tetrahydrofolate
+
electron-transfer flavoprotein
=
sarcosine
+
5,10-methylenetetrahydrofolate
+
reduced electron-transfer flavoprotein

Synonyms

Csal_0990, DdhC, DMGDH, EC 1.5.99.2, Me2GlyDH, mMe2GlyDH, N,N-dimethylglycine oxidase, pMe2GlyDH

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.8 With a flavin or flavoprotein as acceptor
                1.5.8.4 dimethylglycine dehydrogenase

Purification

Purification on EC 1.5.8.4 - dimethylglycine dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme from liver mitochondria, recombinant His-tagged enzyme from Escherichia coli 45fold by nickel affinity chromatography
-
Ni-Sepharose 6 column chromatography and MonoQ column chromatography
Ni2+-Sepharose column chromatography
of in vitro synthesized 6-His-dimethylglycine dehydrogenase on nickel chelating Sepharose
-
using ammonium sulfate treatment and dissolution in 7.5 mM potassium phosphate
-
using gel filtration chromatography on Sephadex G-150, DEAE-cellulose chromatography, and affinity chromatography
-