1.5.7.1: methylenetetrahydrofolate reductase (ferredoxin)
This is an abbreviated version!
For detailed information about methylenetetrahydrofolate reductase (ferredoxin), go to the full flat file.
Reaction
Synonyms
5,10-methylenetetrahydrofolate reductase, Fdred:methylene-THF oxidoreductase, metF, methylene-H4F reductase, methylene-tetrahydrofolate reductase, methylenetetrahydrofolate reductase, MetVF-type methylenetetrahydrofolate reductase, MetVF-type MTHFR, MTHFR
ECTree
Advanced search results
Purification
Purification on EC 1.5.7.1 - methylenetetrahydrofolate reductase (ferredoxin)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
native enzyme 57.6fold from cytoplasm by anion exchange chromatography, hydrophobic interaction chromatography, ultrafiltration, and gel filtration, to homogeneity
-
native enzyme aerobically 40fold by ultracentrifugation, anion exchange chromatography, gel filtration, ultrafiltration, hydroxyapatite chromatography, followed by again ultrafiltration and gel filtration, enzyme MetF copurifies with the other five proteins encoded in the unit in a hexaheteromeric complex with an apparent molecular mass in the 320-kDa range. Recombinant Strep-tagged enzyme MetF anaerobically from Escherichia coli strain C41(DE3) by avidine affinity chromatography and ultrafiltration
-