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1.5.3.6: (R)-6-hydroxynicotine oxidase

This is an abbreviated version!
For detailed information about (R)-6-hydroxynicotine oxidase, go to the full flat file.

Word Map on EC 1.5.3.6

Reaction

(R)-6-hydroxynicotine
+
H2O
 +
O2
=
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one
 +
H2O2

Synonyms

6-HDNO, 6-hydroxy-D-nicotine oxidase, D-6-hydroxynicotine oxidase

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.3 With oxygen as acceptor
                1.5.3.6 (R)-6-hydroxynicotine oxidase

Engineering

Engineering on EC 1.5.3.6 - (R)-6-hydroxynicotine oxidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C136S
-
5% of wild-type activity, slight activation with glycerol-3-phosphate and FAD
C136S/C260S
-
no activity, no activation with glycerol-3-phosphate and FAD
C260S
-
15% of wild-type activity, no activation with glycerol-3-phosphate and FAD
C433S
C59S
-
40% of wild-type activity, no activation with glycerol-3-phosphate and FAD
E350A
Q8GAG1
substitution decreases the kcat/Kamine value by about 20fold
E350Q
Q8GAG1
substitution decreases the kcat/Kamine value by about 220fold
E352Q
Q8GAG1
substitution decreases the kcat/Kamine value about 3800fold
K348M
Q8GAG1
substitution has only a small effect on the kinetic parameters
P73A
-
unable to bind FAD
R67A
-
no enzyme activity, no flavinylation
R67K
-
3% activity of wild-type, better flavinylation rate than wild type enzyme
S68A
-
80% activity of wild-type, flavinylation