1.5.3.6: (R)-6-hydroxynicotine oxidase
This is an abbreviated version!
For detailed information about (R)-6-hydroxynicotine oxidase, go to the full flat file.
Word Map on EC 1.5.3.6
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1.5.3.6
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fad
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arthrobacter
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oxidans
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flavinylation
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holoenzyme
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flavin
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flavoproteins
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6-hydroxy-l-nicotine
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flavoenzymes
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apoenzyme
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nicotinovorans
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nicotine-degrading
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l-nicotine
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dimethylglycine
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glycerol-3-p
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cotranslational
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fad-binding
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l-enantiomer
- 1.5.3.6
- fad
- arthrobacter
- oxidans
-
flavinylation
-
holoenzyme
- flavin
- flavoproteins
- 6-hydroxy-l-nicotine
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flavoenzymes
-
apoenzyme
- nicotinovorans
-
nicotine-degrading
- l-nicotine
- dimethylglycine
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glycerol-3-p
-
cotranslational
-
fad-binding
-
l-enantiomer
Reaction
Synonyms
6-HDNO, 6-hydroxy-D-nicotine oxidase, D-6-hydroxynicotine oxidase
ECTree
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Cloned
Cloned on EC 1.5.3.6 - (R)-6-hydroxynicotine oxidase
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expressed in Escherichia coli HB101 as a 6-hydroxy-D-nicotine oxidase/glutathione S-transferase fusion protein
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expressed in Escherichia coli JM109 as a beta-galactosidase/6-hydroxynicotine oxidase fusion protein
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expressed in Escherichia coli JM109 as a fusion protein with dimethylglycine dehydrogenase and translated into rabbit reticulocyte lysate
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expressed in Saccharomyces cerevisiae as a fusion protein between 6-hydroxy-D-nicotine oxidase and the mitochondrial targeting sequence of Neurospora crassa F0-ATPase subunit 9 (Su9-6-HDNO) and translated into rabbit reticulocyte lysate
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expression is temperature dependent, higher specific activities are found at 30°C than at 37°C
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