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1.5.3.6: (R)-6-hydroxynicotine oxidase

This is an abbreviated version!
For detailed information about (R)-6-hydroxynicotine oxidase, go to the full flat file.

Word Map on EC 1.5.3.6

Reaction

(R)-6-hydroxynicotine
+
H2O
+
O2
=
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one
+
H2O2

Synonyms

6-HDNO, 6-hydroxy-D-nicotine oxidase, D-6-hydroxynicotine oxidase

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.3 With oxygen as acceptor
                1.5.3.6 (R)-6-hydroxynicotine oxidase

Cloned

Cloned on EC 1.5.3.6 - (R)-6-hydroxynicotine oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli HB101
-
expressed in Escherichia coli HB101 as a 6-hydroxy-D-nicotine oxidase/glutathione S-transferase fusion protein
-
expressed in Escherichia coli JM101
-
expressed in Escherichia coli JM109
-
expressed in Escherichia coli JM109 as a beta-galactosidase/6-hydroxynicotine oxidase fusion protein
-
expressed in Escherichia coli JM109 as a fusion protein with dimethylglycine dehydrogenase and translated into rabbit reticulocyte lysate
-
expressed in Escherichia coli K12 strain HB101, maxicells
-
expressed in Escherichia coli S30 wild type and mutants
-
expressed in Saccharomyces cerevisiae as a fusion protein between 6-hydroxy-D-nicotine oxidase and the mitochondrial targeting sequence of Neurospora crassa F0-ATPase subunit 9 (Su9-6-HDNO) and translated into rabbit reticulocyte lysate
-
expression in Escherichia coli
-
expression is temperature dependent, higher specific activities are found at 30°C than at 37°C
-