1.5.3.1: sarcosine oxidase (formaldehyde-forming)
This is an abbreviated version!
For detailed information about sarcosine oxidase (formaldehyde-forming), go to the full flat file.
Word Map on EC 1.5.3.1
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1.5.3.1
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flavin
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fad
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heterotetrameric
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n-methylglycine
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creatininase
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amidinohydrolase
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n-methyltryptophan
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corynebacterial
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flavinylation
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diagnostics
-
analysis
-
medicine
- 1.5.3.1
- flavin
- fad
-
heterotetrameric
- n-methylglycine
- creatininase
-
amidinohydrolase
- n-methyltryptophan
-
corynebacterial
-
flavinylation
- diagnostics
- analysis
- medicine
Reaction
Synonyms
heterotetrameric sarcosine oxidase, L-pipecolate oxidase, L-pipecolic acid oxidase, monomeric sarcosine oxidase, MSOX, PSO, sarcosine : oxygen oxidoreductase (demethylating), sarcosine oxidase, sarcosine: O2 oxidoreductase, sarcosine:oxygen oxidoreductase (demethylating), SO, SO-U96, SOX, SoxA, trd_1773, TSOX
ECTree
1.5.3.1 sarcosine oxidase (formaldehyde-forming)Advanced search results
results (
results (Substrates Products
Substrates Products on EC 1.5.3.1 - sarcosine oxidase (formaldehyde-forming)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-isopropyl-4-(methylaminomethyl)thiazole + O2 + H2O
formaldehyde + 2-isopropyl-4-(aminomethyl)thiazole + H2O2
L-proline + O2 + H2O
?
-
less than 1% the rate of sarcosine
-
-
r
N-methyl-D-proline + O2 + H2O
formaldehyde + D-proline + H2O2
-
-
-
?
N-methyl-L-aspartate + O2 + H2O
formaldehyde + L-aspartate + H2O2
-
-
-
?
N-methyl-L-phenylalanine + O2 + H2O
formaldehyde + L-phenylalanine + H2O2
-
-
-
?
sarcosine + H2O + 2,6-dichlorophenolindophenol
glycine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
-
r
sarcosine + H2O + phenazine methosulfate
glycine + formaldehyde + reduced phenazine methosulfate
-
-
-
-
r
sarcosine + H2O + potassium ferricyanide
glycine + formaldehyde + potassium ferrocyanide
-
-
-
-
r
2-isopropyl-4-(methylaminomethyl)thiazole + O2 + H2O
formaldehyde + 2-isopropyl-4-(aminomethyl)thiazole + H2O2
-
-
-
?
2-isopropyl-4-(methylaminomethyl)thiazole + O2 + H2O
formaldehyde + 2-isopropyl-4-(aminomethyl)thiazole + H2O2
-
-
-
?
4-(ethylaminomethyl)pyridine + O2 + H2O
acetaldehyde + 4-(aminomethyl) pyridine + H2O2
-
-
-
?
4-(ethylaminomethyl)pyridine + O2 + H2O
acetaldehyde + 4-(aminomethyl) pyridine + H2O2
-
-
-
?
N-methyl-DL-alanine + O2 + H2O
formaldehyde + DL-alanine + H2O2
-
-
-
-
?
N-methyl-DL-alanine + O2 + H2O
formaldehyde + DL-alanine + H2O2
-
-
-
-
?
N-methyl-DL-alanine + O2 + H2O
formaldehyde + DL-alanine + H2O2
Bacillus sp. (in: Bacteria) B-0618
-
-
-
-
?
N-methyl-DL-alanine + O2 + H2O
formaldehyde + DL-alanine + H2O2
-
-
-
-
?
N-methyl-DL-alanine + O2 + H2O
formaldehyde + DL-alanine + H2O2
-
-
-
-
?
N-methyl-DL-alanine + O2 + H2O
formaldehyde + DL-alanine + H2O2
-
-
-
-
?
N-methyl-DL-valine + O2 + H2O
formaldehyde + DL-valine + H2O2
-
-
-
-
?
N-methyl-DL-valine + O2 + H2O
formaldehyde + DL-valine + H2O2
Bacillus sp. (in: Bacteria) B-0618
-
-
-
-
?
N-methyl-DL-valine + O2 + H2O
formaldehyde + DL-valine + H2O2
-
-
-
-
?
N-methyl-L-alanine + O2 + H2O
formaldehyde + L-alanine + H2O2
-
-
-
?
N-methyl-L-alanine + O2 + H2O
formaldehyde + L-alanine + H2O2
-
-
-
?
N-methyl-L-leucine + O2 + H2O
formaldehyde + L-leucine + H2O2
-
-
-
-
?
N-methyl-L-leucine + O2 + H2O
formaldehyde + L-leucine + H2O2
Bacillus sp. (in: Bacteria) B-0618
-
-
-
-
?
N-methyl-L-leucine + O2 + H2O
formaldehyde + L-leucine + H2O2
-
-
-
-
?
N-methyl-L-leucine + O2 + H2O
formaldehyde + L-leucine + H2O2
-
-
-
?
N-methyl-L-leucine + O2 + H2O
formaldehyde + L-leucine + H2O2
-
-
-
?
N-methyl-L-tryptophan + O2 + H2O
formaldehyde + L-tryptophan + H2O2
-
-
-
?
N-methyl-L-tryptophan + O2 + H2O
formaldehyde + L-tryptophan + H2O2
-
-
-
?
sarcosine + 5,6,7,8-tetrahydrofolate + O2
glycine + formaldehyde + H2O2
-
-
-
-
?
sarcosine + 5,6,7,8-tetrahydrofolate + O2
glycine + formaldehyde + H2O2
-
-
-
?
sarcosine + H2O + O2
?
-
sarcosine degradation when sarcosine is sole source of carbon, nitrogen and energy
-
-
?
sarcosine + H2O + O2
glycine + formaldehyde + H2O2
-
-
-
-
?
sarcosine + H2O + O2
glycine + formaldehyde + H2O2
-
-
-
?
sarcosine + H2O + O2
glycine + formaldehyde + H2O2
-
the simulation method of Markovian milestoning molecular dynamics simulations is used to compute the entry and exit kinetics of O2 in the enzyme. The rate of flavin oxidation by O2 is likely not strongly limited by diffusion from the solvent to the active site. The predicted faster entry and slower exit of O2 for the bound state indicate a longer residence time within the enzyme, increasing the likelihood of collisions with the flavin isoalloxazine ring, a step required for reduction of molecular O2 and subsequent reoxidation of the flavin
-
-
?
sarcosine + H2O + O2
glycine + formaldehyde + H2O2
-
-
-
-
?
sarcosine + H2O + O2
glycine + formaldehyde + H2O2
-
-
-
?
sarcosine + H2O + O2
glycine + formaldehyde + H2O2
-
-
-
?
sarcosine + H2O + O2
glycine + formaldehyde + H2O2
molecular dynamics investigation by random acceleration molecular dynamics simulations with an ensemble made of the bacterial monomeric sarcosine oxidase (2GF3), O2, and the inhibitor furoic acid to mimic sarcosine. The ensemble is solvated by in a periodic box, while an external tiny force acts randomly to expel O2 from the center of activation, located between residue K265 and the si face of the flavin ring of the flavin adenine dinucleotide cofactor, moving it toward the solvent
-
-
?
sarcosine + H2O + O2
glycine + formaldehyde + H2O2
-
-
-
?
sarcosine + H2O + O2
glycine + formaldehyde + H2O2
-
-
-
?
sarcosine + O2 + H2O
formaldehyde + glycine + H2O2
-
-
-
?
sarcosine + O2 + H2O
formaldehyde + glycine + H2O2
-
-
-
?
sarcosine + O2 + H2O
glycine + formaldehyde + H2O2
-
-
-
?
sarcosine + O2 + H2O
glycine + formaldehyde + H2O2
-
-
-
?
sarcosine + O2 + H2O
glycine + formaldehyde + H2O2
-
-
-
?
sarcosine + O2 + H2O
glycine + formaldehyde + H2O2
-
-
-
?
additional information
?
-
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not: beta-alanine, N-methylalanine, 1,3-dimethylurea, 1-methylguanidine, methoxyacetate, creatine, creatinine
-
-
?
additional information
?
-
also oxidizes other amino acids containing a secondary amino group e.g. L-proline, and N-methyl-L-alanine
-
-
?
additional information
?
-
Bacillus sp. (in: Bacteria) B-0618
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not: beta-alanine, N-methylalanine, 1,3-dimethylurea, 1-methylguanidine, methoxyacetate, creatine, creatinine
-
-
?
additional information
?
-
-
flavin and cytochromes of the c and b or o type function as electron carriers
-
-
?
additional information
?
-
-
very specific for oxygen as acceptor, oxygen can be replaced by 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, much smaller Vmax/Km values than for O2
-
-
?
additional information
?
-
-
heterotetrameric sarcosine oxidase is a flavoprotein that catalyses the oxidative demethylation of sarcosine to generate glycine, hydrogen peroxide and formaldehyde or 5,10-methylenetetrahydrofolate, depending on the availability of tetrahydrofolate. The amine proton of sarcosine is transferred to the unprotonated Lys residue in the enzyme-substrate complex
-
-
?
additional information
?
-
-
no substrate: choline,betaine, dimethylglycine and N-methyl amino acids
-
-
?
additional information
?
-
-
no substrate: choline,betaine, dimethylglycine and N-methyl amino acids
-
-
?
additional information
?
-
-
the alpha subunit shows sarcosine oxidase and L-proline dehydrogenase activity, while the beta subunit displays both sarcosine oxidase and L-proline dehydrogenase activity, but not NADH dehydrogenase activity, measurement of L-proline dehydrogenase activity with 2,6-dichloroindophenol as cofactor. Dye-linked NADH dehydrogenase activity is assayed with NADH, FAD and 2,6-dichloroindophenol
-
-
?
