1.5.3.1: sarcosine oxidase (formaldehyde-forming)

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For detailed information about sarcosine oxidase (formaldehyde-forming), go to the full flat file.

Word Map on EC 1.5.3.1

1.5.3.1

flavin

fad

heterotetrameric

n-methylglycine

creatininase

amidinohydrolase

n-methyltryptophan

corynebacterial

flavinylation

diagnostics

analysis

medicine

Reaction

Synonyms

heterotetrameric sarcosine oxidase, L-pipecolate oxidase, L-pipecolic acid oxidase, monomeric sarcosine oxidase, MSOX, PSO, sarcosine : oxygen oxidoreductase (demethylating), sarcosine oxidase, sarcosine: O2 oxidoreductase, sarcosine:oxygen oxidoreductase (demethylating), SO, SO-U96, SOX, SoxA, trd_1773, TSOX

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.3 With oxygen as acceptor

1.5.3.1 sarcosine oxidase (formaldehyde-forming)

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Results	in table
7443	AA Sequence
10	Application
1	CAS Registry Number
15	Cloned(Commentary)
48	Cofactor
11	Crystallization (Commentary)
3	General Information
1	General Stability
5	IC50 Value
83	Inhibitors
22	kcat/KM [mM/s]
63	KM Value [mM]
2	Localization
3	Metals/Ions
33	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
100	Organism
7	Pathway
55	PDB ID
13	pH Optimum
8	pH Range
7	pH Stability
48	Protein Variants
22	Purification (Commentary)
17	Reaction
4	Reaction Type
90	Reference
2	Renatured (Commentary)
4	Source Tissue
15	Specific Activity [micromol/min/mg]
3	Storage Stability
112	Substrates and Products (Substrate)
32	Subunits
31	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
10	Temperature Stability [°C]
44	Turnover Number [1/s]

Purification

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Purification on EC 1.5.3.1 - sarcosine oxidase (formaldehyde-forming)

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6 entries

affinity matrix is 5-formyltetrahydrofolate coupled to AH-sepharose

Corynebacterium sp.

392394

affinity-purification, 4-aminopyrrole-2-carboxylic acid as the affinity ligand, which is coupled with Sepharose CL 4B via spacers composed of epichlorohydrin and ethylenediamine. With the affinity medium, the purification process consists of only one affinity chromatography step to capture monomeric enzyme. The purified enzyme iss 94% and 96% pure when analyzed on an HPLC and reducing SDS-PAGE

Bacillus sp. BSD-8

Q6ITC6

743178

DEAEcellulose, QAE-Sephadex, and Ultrogel column chromatographies

Corynebacterium sp. U-96

764086

HiTrap DEAE column chromatography, HisTrap column chromatography, HiTrap phenyl column chromatography, Resources Q column chromatography, and Superdex 200 gel filtration

Corynebacterium sp. U-96

764062

native enzyme by ammoniumm sulfate fractionation, hydrophobic interaction and anion exchange chromatography, and gel filtration

Bacillus sp. (in: Bacteria)

724775

P-1

Corynebacterium sp.

392391

recombinant His-tagged alpha and beta subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity

Thermococcus kodakarensis

724937

recombinant native and mutant enzyme

Bacillus sp. (in: Bacteria)

P40859

685219

recombinant protein

Bacillus sp. (in: Bacteria)

Q6ITC6

671547

recombinant protein, purified by one step of affinity purification on a Ni-Sepharose column, and another step of ion-exchange purification on a DEAE column

Thermomicrobium roseum

B9L163

765378

recombinant proteins

Bacillus sp. (in: Bacteria)

685194

recombinant proteins using His-tag

Bacillus sp. (in: Bacteria)

685282

straight gram-negative rod

to homogeneity

U-96