1.5.3.1: sarcosine oxidase (formaldehyde-forming)

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For detailed information about sarcosine oxidase (formaldehyde-forming), go to the full flat file.

Reaction

Synonyms

heterotetrameric sarcosine oxidase, L-pipecolate oxidase, L-pipecolic acid oxidase, monomeric sarcosine oxidase, MSOX, PSO, sarcosine : oxygen oxidoreductase (demethylating), sarcosine oxidase, sarcosine: O2 oxidoreductase, sarcosine:oxygen oxidoreductase (demethylating), SO, SO-U96, SOX, SoxA, trd_1773, TSOX

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.3 With oxygen as acceptor

                1.5.3.1 sarcosine oxidase (formaldehyde-forming)

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Results	in table
7443	AA Sequence
10	Application
1	CAS Registry Number
15	Cloned(Commentary)
48	Cofactor
11	Crystallization (Commentary)
3	General Information
1	General Stability
5	IC50 Value
83	Inhibitors
22	kcat/KM [mM/s]
63	KM Value [mM]
2	Localization
3	Metals/Ions
33	Molecular Weight [Da]
24	Natural Substrates/ Products (Substrates)
100	Organism
7	Pathway
55	PDB ID
13	pH Optimum
8	pH Range
7	pH Stability
48	Protein Variants
22	Purification (Commentary)
17	Reaction
4	Reaction Type
90	Reference
2	Renatured (Commentary)
4	Source Tissue
15	Specific Activity [micromol/min/mg]
3	Storage Stability
112	Substrates and Products (Substrate)
32	Subunits
31	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
10	Temperature Stability [°C]
44	Turnover Number [1/s]

General Information

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General Information on EC 1.5.3.1 - sarcosine oxidase (formaldehyde-forming)

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

metabolism

Bacillus sp. B-0618

P40859

hydride-transfer is the most energetically favorable mechanism. Hydrogen is not transferred in the hydride state (H-) but in a hydrogen atom state. A single electron is simultaneously transferred from sarcosine to flavin through their overlapping orbitals, the reaction mechanism can be labeled the hydrogen-atom coupled electron-transfer (HACET) mechanism. Sarcosine is moved close to the flavin ring because of a small charge transfer and the positively charged residues (Arg49, Arg52, and Lys348) near the active site play a prominent role in stabilizing the sarcosine-flavin complex

765533

physiological function

Thermococcus kodakarensis

-

sarcosine oxidase catalyzes the oxidation of the methyl group in sarcosine and transfer of the oxidized methyl group into the one-carbon metabolic pool, yielding equimolar glycine, hydrogen peroxide, and formaldehyde or 5,10-methylenetetrahydrofolate

724937

additional information

Thermococcus kodakarensis

-

the recombinant alpha subunit forms a dimeric structure and behaves as an NADH dehydrogenase, while the beta subunit is a tetramer that has sarcosine oxidase and L-proline dehydrogenase activity.. The SOX complex assembles into the hetero-octameric (alphabeta)4 form and shows NADH dehydrogenase activity, structure of isolated subunit tetramers, overview

724937