1.5.1.B5: DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase (NADH)
This is an abbreviated version!
For detailed information about DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase (NADH), go to the full flat file.
Word Map on EC 1.5.1.B5
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1.5.1.B5
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sulfatases
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mucopolysaccharidosis
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formylglycine-generating
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metachromatic
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sulfatase-modifying
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leukodystrophy
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ichthyosis
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formylglycine
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aav-mediated
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x-linked
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co-delivery
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intracerebral
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infantile
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autosomal-recessive
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ataxia
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mpsiiia
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adeno-associated
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posttranslationally
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sulfamidase
- 1.5.1.B5
-
sulfatases
-
mucopolysaccharidosis
-
formylglycine-generating
-
metachromatic
-
sulfatase-modifying
-
leukodystrophy
-
ichthyosis
-
formylglycine
-
aav-mediated
-
x-linked
-
co-delivery
-
intracerebral
-
infantile
-
autosomal-recessive
-
ataxia
-
mpsiiia
-
adeno-associated
-
posttranslationally
-
sulfamidase
Reaction
Synonyms
DELTA-pyrroline-2-carboxylate reductase, DELTA1-pyrroline-2-carboxylate reductase, NADH-dependent Pyr2C reductase, Pyr2C reductase, TlLhpI, tlPyr2C
ECTree
Advanced search results
Engineering
Engineering on EC 1.5.1.B5 - DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase (NADH)
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L52E/V224D/A228K
L52R/V224D/A228K
V224D/A228K
L52E/V224D/A228K
L52R/V224D/A228K
V224D/A228K
additional information
H3ZMH3; H3ZMH4
DELTA1-pyrroline-2-carboxylate reductase activity is the same as in wild-type enzyme, no alanine dehydrogenase activity
L52E/V224D/A228K
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site-directed mutagenesis, the mutant shows 2fold increased activity and highly increased specifiicity for substrate DELTA1-pyrrolidine-2-carboxylate as compared to the wild-type enzyme
H3ZMH3; H3ZMH4
DELTA1-pyrroline-2-carboxylate reductase activity is the same as in wild-type enzyme, no alanine dehydrogenase activity
L52R/V224D/A228K
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site-directed mutagenesis, the mutant shows slightly increased activity and highly increased specifiicity for substrate DELTA1-pyrrolidine-2-carboxylate and no activity with other substrates in contrast to the wild-type enzyme
H3ZMH3; H3ZMH4
12fold enhancement of DELTA1-pyrroline-2-carboxylate reductase activity, 6.7fold enhancement for the oxidization activity for L-proline, no alanine dehydrogenase activity
V224D/A228K
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site-directed mutagenesis, the mutant shows 10fold increased activity for substrate DELTA1-pyrrolidine-2-carboxylate as compared to the wild-type enzyme
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site-directed mutagenesis, the mutant shows 2fold increased activity and highly increased specifiicity for substrate DELTA1-pyrrolidine-2-carboxylate as compared to the wild-type enzyme
-
L52E/V224D/A228K
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DELTA1-pyrroline-2-carboxylate reductase activity is the same as in wild-type enzyme, no alanine dehydrogenase activity
-
-
site-directed mutagenesis, the mutant shows slightly increased activity and highly increased specifiicity for substrate DELTA1-pyrrolidine-2-carboxylate and no activity with other substrates in contrast to the wild-type enzyme
-
L52R/V224D/A228K
-
DELTA1-pyrroline-2-carboxylate reductase activity is the same as in wild-type enzyme, no alanine dehydrogenase activity
-
-
site-directed mutagenesis, the mutant shows 10fold increased activity for substrate DELTA1-pyrrolidine-2-carboxylate as compared to the wild-type enzyme
-
V224D/A228K
-
12fold enhancement of DELTA1-pyrroline-2-carboxylate reductase activity, 6.7fold enhancement for the oxidization activity for L-proline, no alanine dehydrogenase activity
-
H3ZMH3; H3ZMH4
TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consistsof two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
additional information
-
TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consistsof two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
additional information
-
TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consistsof two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
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