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1.5.1.7: saccharopine dehydrogenase (NAD+, L-lysine-forming)

This is an abbreviated version!
For detailed information about saccharopine dehydrogenase (NAD+, L-lysine-forming), go to the full flat file.

Reaction

N6-(L-1,3-dicarboxypropyl)-L-lysine
+
NAD+
+
H2O
=
L-lysine
+
2-oxoglutarate
+
NADH
+
H+

Synonyms

dehydrogenase, saccharopine (nicotinamide adenine dinucleotide, lysine forming), epsilon-N-(L-glutaryl-2)-L-lysine:NAD oxidoreductase (L-lysine forming), Fvsdh, LYS1, Lys1p, Lysine--2-oxoglutarate reductase, lysine-2-oxoglutarate reductase, N6-(glutar-2-yl)-L-lysine:NAD oxidoreductase (L-lysine-forming), N6-(glutaryl-2)-L-lysine: NAD oxidoreductase, N6-(glutaryl-2)-L-lysine: NAD+ oxidoreductase, N6-(glutaryl-2)-l-lysine:NAD oxidoreductase, N6-(glutaryl-2)-L-lysine:NAD oxidoreductase (L-lysine forming), N6-(glutaryl-2)-L-lysine:NAD-oxidoreductase (L-lysine-forming), N6-(glutaryl-2)-L-lysine:nicotinamide adenine dinucleotide (NAD+) oxidoreductase (L-lysine-forming), NAD+-linked saccharopine dehydrogenase, saccharopine dehydrogenase, saccharopine dehydrogenase (L-lysine-forming), SDH

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.7 saccharopine dehydrogenase (NAD+, L-lysine-forming)

Crystallization

Crystallization on EC 1.5.1.7 - saccharopine dehydrogenase (NAD+, L-lysine-forming)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with NAD+ and saccharopine, hanging drop vapour diffusion method in 20% (w/v) PEG 8000, 0.1 M MES (pH 6.0), and 0.2 M Ca(OAc)2
mutant enzyme C205S, hanging drop vapor diffusion method, using 100 mM Tris (pH 7.0), 30% (w/v) PEG-MME 2000 at 4°C
the apo-enzyme is obtained using the hanging drop vapour diffusion method with 100 mM Tris (pH 7.0) and 22% (w/v) PEG 8000, sulfate-, AMP-, and oxalylglycine-bound enzyme crystals are obtained by the hanging drop vapour diffusion method with 26% (w/v) PEG-MME 2000 in 100 mM Tris (pH 7.0) containing 200 mM (NH4)2SO4, or with 24% (w/v) PEG-MME 2000 in 100 mM Bis-Tris, pH 6.5, or with 24% (w/v) PEG-MME 2000 in 100 mM Tris, pH 8.0, at 4°C, respectively