1.5.1.40: 8-hydroxy-5-deazaflavin:NADPH oxidoreductase
This is an abbreviated version!
For detailed information about 8-hydroxy-5-deazaflavin:NADPH oxidoreductase, go to the full flat file.
Word Map on EC 1.5.1.40
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1.5.1.40
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methanogen
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archaea
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hydride
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8-hydroxy-5-deazaflavins
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methanococcus
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hydrogenase
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vannielii
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thermoautotrophicum
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methanobacterium
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methanobrevibacter
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griseus
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methanogenesis
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stereochemical
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smithii
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si-face
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methane
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fulgidus
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ch4
- 1.5.1.40
-
methanogen
- archaea
-
hydride
- 8-hydroxy-5-deazaflavins
-
methanococcus
- hydrogenase
- vannielii
- thermoautotrophicum
-
methanobacterium
-
methanobrevibacter
- griseus
-
methanogenesis
-
stereochemical
- smithii
-
si-face
- methane
- fulgidus
- ch4
Reaction
Synonyms
5-deazaflavin-NADP+ reductase, 8-hydroxy-5-deazaflavin-dependent NADP+ reductase, 8-OH-5-deazaflavin:NADPH oxidoreductase, 8-OH-5dFl:NADPH oxidoreductase, AF0892, F420-dependent NADP oxidoreductase, F420-dependent NADP reductase, F420-dependent NADP+ oxidoreductase, F420:NADPH oxidoreductase, F420H2:NADP oxidoreductase, F420H2:NADP+ oxidoreductase, Fno, Msm_0049, NADP+:F420 oxidoreductase, Tfu-FNO, Tfu_0970
ECTree
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Purification
Purification on EC 1.5.1.40 - 8-hydroxy-5-deazaflavin:NADPH oxidoreductase
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recombinant enzyme from cell-free extracts of Escherichia coli strain C41(DE3) by heat treatment at 90°C for 30 min, ammonium sulfate fractionation with addition of 0.05% polyethylenimine, followed by an ion exchange chromatography, ultrafiltration, and gel filtration
recombinant enzyme from Escherichia coli by ammonium sulfate fractionation followed by anion exchange chromatography, DNase I treatment during protein purification is essential to remove residual DNA
recombinant wild-type and mutant enzymes from cell-free extracts of Escherichia coli strain C41(DE3) by heat treatment at 90°C for 30 min, ammonium sulfate fractionation with addition of 0.05% polyethylenimine, followed by an ion exchange chromatography, ultrafiltration, and gel filtration