1.5.1.38: FMN reductase (NADPH)

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For detailed information about FMN reductase (NADPH), go to the full flat file.

Reaction

Synonyms

(NADPH)-dependent flavin mononucleotide reductase, (NADPH)-dependent FMN reductase, BC_1619, EC 1.5.1.29, EC 1.6.8.1, flavin reductase P, FMN reductase, FRP, More, NAD(P)H:FMN reductase, NADPH specific FMN reductase, NADPH-flavin oxidoreductase, NADPH-FMN oxidoreductase, NADPH:FMN oxidoreductase, NADPH:FMN reductase, SsuE, ssueE, ycbP, ydgI

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.1 With NAD⁺ or NADP⁺ as acceptor

1.5.1.38 FMN reductase (NADPH)

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Results	in table
4073	AA Sequence
7	Cloned(Commentary)
16	Cofactor
3	Crystallization (Commentary)
11	General Information
9	Inhibitors
13	kcat/KM [mM/s]
28	KM Value [mM]
18	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
17	Organism
4	Pathway
31	PDB ID
8	pH Optimum
1	pH Range
10	Protein Variants
10	Purification (Commentary)
6	Reaction
17	Reference
1	Renatured (Commentary)
3	Specific Activity [micromol/min/mg]
2	Storage Stability
36	Substrates and Products (Substrate)
12	Subunits
42	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
1	Temperature Stability [°C]
8	Turnover Number [1/s]

Purification

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Purification on EC 1.5.1.38 - FMN reductase (NADPH)

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Vibrio harveyi

392179, 392180, 392297, 392300

DEAE-Sepharose column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration

recombinant enzyme

recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography and desalting gel filtration

Bacillus cereus

Q81BW8

741865

recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)

Escherichia coli

P80644

741937, 764152

SsuE is purified to homogeneity as an N-terminal histidine-tagged fusion protein

Escherichia coli

P80644

438770