1.5.1.36: flavin reductase (NADH)

This is an abbreviated version!
For detailed information about flavin reductase (NADH), go to the full flat file.

Word Map on EC 1.5.1.36

1.5.1.36

nadph:flavin

fmnh2

alkanesulfonate

oxygen-insensitive

beneckea

desulfonation

nitroreductases

fmnh2-dependent

Reaction

Synonyms

AbeF, BaiH, BorF, C1-HpaH, DszD, FAD reductase, FerA, flavin mononucleotide reductase, flavin reductase, flavin:NADH oxidoreductase, FMN reductase, Frd188, frd2, fre, HpaC, LJ0548, LJ0549, LJ_0548, LJ_0549, LuxG, More, NAD(P)H-dependent H2O2-forming flavin reductase, NAD(P)H-flavin oxidoreductase, NAD(P)H:flavin oxidoreductase, NAD(P)H:flavin-oxidoreductase, NADH-dependent flavin reductase, NADH-flavin oxidoreductase, NADH: flavin oxidoreductase, NADH: flavinoxidore ductase/NADH oxidase, NADH:flavin oxidoreductase, NADH:FMN oxidoreductase, nfr1, nfr2, NOX, Pden2689, SMOB-ADP1

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.1 With NAD⁺ or NADP⁺ as acceptor

1.5.1.36 flavin reductase (NADH)

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Results	in table
8	Activating Compound
1935	AA Sequence
6	Application
19	Cloned(Commentary)
34	Cofactor
3	Crystallization (Commentary)
5	Expression
19	General Information
35	Inhibitors
7	kcat/KM [mM/s]
4	Ki Value [mM]
28	KM Value [mM]
2	Localization
6	Metals/Ions
12	Molecular Weight [Da]
30	Natural Substrates/ Products (Substrates)
66	Organism
2	Pathway
4	PDB ID
11	pH Optimum
3	pH Range
2	pI Value
16	Protein Variants
16	Purification (Commentary)
4	Reaction
40	Reference
2	Renatured (Commentary)
3	Source Tissue
10	Specific Activity [micromol/min/mg]
2	Storage Stability
75	Substrates and Products (Substrate)
33	Subunits
84	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
1	Temperature Stability [°C]
11	Turnover Number [1/s]

Purification

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Purification on EC 1.5.1.36 - flavin reductase (NADH)

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2 entries

ammonium sulfate precipitation, butyl-Sepharose column chromatography, Source 30Q column chromatography, FMN-Sepharose 6B affinity column chromatography, and Superdex 75 gel filtration

Mycobacterium goodii

726414

ammonium sulfate precipitation, HiTrap Q column chromatography, and Superdex 200 gel filtration

Lactobacillus acidophilus

Q74HL7, Q74HL8

741680

native enzyme from strain NCC 533 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration

Lactobacillus johnsonii

Q74HL7; Q74HL8

741680

Ni-affinity chromatography and heat treatment

Sulfurisphaera tokodaii

720976

Ni2+ column chromatography

Pseudomonas aeruginosa

711238

recombinant AbeF from Escherichia coli

uncultured bacterium AB1650

F6LWA7

764038

recombinant BorF from Escherichia coli

uncultured bacterium AB1650

F6LWA7

764038

recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography

Paracoccus denitrificans

A1B5I2

743241

recombinant enzyme produced in Escherichia coli K-12 cells

Escherichia coli W

Q57501

438899

recombinant His-tagged wild-type enzymes HpaC and Fre from strain BL21(DE3)

Escherichia coli

658006

recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Corynebacterium crenatum

743224

refolded recombinant enzyme by anion exchange chromatography and gel filtration, or by nickel affinity chromatography and gel filtration, when a His10-tagged enzyme is expressed

Acinetobacter baylyi

Q6F936

741794

using Ni-NTA chromatography

Pseudomonas putida

712723