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1.5.1.34: 6,7-dihydropteridine reductase

This is an abbreviated version!
For detailed information about 6,7-dihydropteridine reductase, go to the full flat file.

Word Map on EC 1.5.1.34

Reaction

a 5,6,7,8-tetrahydropteridine
+
NAD(P)+
=
a 6,7-dihydropteridine
 +
NAD(P)H
 +
H+

Synonyms

7,8-dihydrobiopterin reductase, BmDhpr, DHPR, dicDHPR, dihydropteridine reductase, dihydropteridine reductase (NADH), DQPR gene product, EC 1.6.99.10, EC 1.6.99.7, EcDHPR, More, NADH-dihydropteridine reductase, NADPH-dihydropteridine reductase, NADPH-specific dihydropteridine reductase, NfsB, NprA nitroreductase, PTR2, QDPR, quinoid dihydropteridine reductase, reductase, dihydropteridine (reduced nicotinamide adenine dinucleotide)

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.34 6,7-dihydropteridine reductase

Purification

Purification on EC 1.5.1.34 - 6,7-dihydropteridine reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ATCC 11299a
-
cells centrifuged, washed with PBS (pH 7.4), resuspended in lysis buffer (50 mM Tris-HCl, pH 7.5), disrupted by sonication, centrifugation, supernatant filtered (0.45 microm), applied to a column with nickel-NTA beads, eluted (50 mM Tris-HCl, pH 7.5), active fractions pooled, concentrated, and exchanged into 50 mM sodium phosphate, pH 6.8, ultrafiltration, applied to a cation-exchange chromatography HS20 column, eluted with salt gradient at pH 6.8, concentration by ultrafiltration, gel-filtration chromatography with Superdex 200 column in 50 mM Tris-HCl, pH 8.0, concentration of eluted fractions in 20 mM Tris-HCl, pH 8.0, by ultrafiltration
-
homogenization of smooth muscle strips in 2-DE lysis buffer containing 8 M urea, 2 M thiourea, 65 mM DTT, 2% CHAPS, and protease inhibitor cocktail, centrifugation, supernatant used for 2-DE and MALDI-TOF/TOF-MS
-
naphthoquinone affinity chromatography, 5'-AMP-Sepharose
-
native enzyme and recombinant wild-type, W104F, A6V and D37I mutant enzyme
-
native enzyme from fifth larval instar of p50 and ah09 strains, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration
-
nickel-affinity column chromatography
recombinant enzyme
-
recombinant enzyme from Escherichia coli by ion exchange chromatography and gel filtration to homogeneity
recombinant enzyme, partially from Escherichia coli, solubilization at pH 10.0
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
recombinant protein using His-tag
-
recombinant wild-type and several mutant enzymes
-
recombinant wild-type, G19D, W104G and T119insert mutant enzymes
-
simple two step procedure i.e. affinity chromatography on Matrex gel blue A and hydrophobic chromatography on phenyl-Sepharose
wild-type and recombinant Y150H, Y150S, Y150F, Y150E, Y150K, G151S and G23D mutant enzymes
-