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1.4.3.2: L-amino-acid oxidase

This is an abbreviated version!
For detailed information about L-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.2

Reaction

an L-amino acid
+
H2O
 +
O2
=
a 2-oxo carboxylate
 +
NH3
 +
H2O2

Synonyms

AAD, ACTX-6, ACTX-8, Akbu-LAAO, APIT, apotxin, aromatic L-amino acid oxidase, Balt-LAAO-I, bordonein-L, Bpir-LAOO-1, CC-LPOX, DAA, Dolabellanin, escapin, head kidney and gill, IL4I1, Il4i1 protein, ink toxin 1, Interleukin Four Induced Gene 1 protein, L-AAO, L-AAO/MOG, L-AAO1, L-amino acid oxidase, L-amino acid: O2 oxidoreductase, L-amino acid:O2 oxidoreductase, L-amino acid:O2 oxidoreductase (deaminating), L-aminooxidase, L-Aox, L-phenylalanine oxidase, LAAO, LAAO-1, LAAO-I, LAAO-II, LAAO1, LAAO4, LAAOI, LAAOII, LAO, LAO1, M-LAO, ophio-amino-acid oxidase, Pm1, RoLAAO, sarA, Sebastes schlegeli antibacterial protein, Sebastes schlegelii antibacterial protein, SSAP, TJ-LAO, toxophallin, TSV-LAO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.2 L-amino-acid oxidase

Temperature Stability

Temperature Stability on EC 1.4.3.2 - L-amino-acid oxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-70
-
the enzyme is stable at low temperatures (-20°C, -70°C) for one week and completely loses its activity after heating at 70°C for 10 min
0 - 50
stable within this range
20 - 60
-
the enzyme has half-life values of 21.2, 8.3, 3.6, 3.1, 2.6 h at 30, 35, 40, 50, 60°C, respectively, and melting temperature of 61.3°C
25
-
1 month, no loss of activity
25 - 50
inactivation in 50 mM triethylamine/HCl buffer, pH 7.6 in the same range as 50 mM potassium-phosphate buffer pH 6.6
30
incubation for 30 min with 0.28 M H2O2 results in 50% inactivation
30 - 50
-
pH 7.0, 60 min, stable up to 30°C, 80% of the enzyme activity remains at 40°C, the enzyme activity is almost completely lost at 50°C
40 - 60
thermal denaturing half-lives of the enzyme at 40, 45, 50, 55 and 60°C are 1855, 642, 332, 105 and 23 min, respectively
40 - 90
-
the enzyme is stable over temperatures up to 40°C although loss in enzyme activity is seen. Treatment of the enzyme at 40 and 50°C for 5 minutes causes 38.47% and 67.19% loss in activity, respectively, compared to the untreated enzyme. When the time of incubation of the enzyme at 40 and 50°C is increased to 10 min, total loss of enzyme activity occurs. Treatment of the enzyme at higher temperatures of 60-90°C for 5 min completely inactivates the enzyme
50 - 60
-
after 120 min incubation, the enzyme retains 80% of its activity at 50°C. At a temperature over 55°C, the activity decreases dramatically to reach 10% at 60°C
50 - 70
the enzyme isoforms are thermally stable up to 70°C for 1 h since no loss of enzymatic activities are recorded up to 40°C for isoform LAAOI and 50°C for LAAOII, whereas 50% loss of the enzymatic activities are recorded at 60°C for isoform LAAOI and 65°C for isoform LAAOII, respectively. On the other hand, a complete loss of activities is detected above 70°C for both isoforms
51
-
5 min, inactivation above 51°C
71
-
5 min, complete inactivation
73
-
no loss of activity after 5 min, heat-stable in presence of 0.01 mM L-leucine
75
-
10 min, 97% loss of activity
additional information