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1.4.3.2: L-amino-acid oxidase

This is an abbreviated version!
For detailed information about L-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.2

Reaction

an L-amino acid
+
H2O
+
O2
=
a 2-oxo carboxylate
+
NH3
+
H2O2

Synonyms

AAD, ACTX-6, ACTX-8, Akbu-LAAO, APIT, apotxin, aromatic L-amino acid oxidase, Balt-LAAO-I, bordonein-L, Bpir-LAOO-1, CC-LPOX, DAA, Dolabellanin, escapin, head kidney and gill, IL4I1, Il4i1 protein, ink toxin 1, Interleukin Four Induced Gene 1 protein, L-AAO, L-AAO/MOG, L-AAO1, L-amino acid oxidase, L-amino acid: O2 oxidoreductase, L-amino acid:O2 oxidoreductase, L-amino acid:O2 oxidoreductase (deaminating), L-aminooxidase, L-Aox, L-phenylalanine oxidase, LAAO, LAAO-1, LAAO-I, LAAO-II, LAAO1, LAAO4, LAAOI, LAAOII, LAO, LAO1, M-LAO, ophio-amino-acid oxidase, Pm1, RoLAAO, sarA, Sebastes schlegeli antibacterial protein, Sebastes schlegelii antibacterial protein, SSAP, TJ-LAO, toxophallin, TSV-LAO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.2 L-amino-acid oxidase

Specific Activity

Specific Activity on EC 1.4.3.2 - L-amino-acid oxidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003
-
cell-free extract, using L-lysine as substrate, at pH 7.0 and 65°C
0.01
-
supernatant from the culture medium
0.24
-
activity against L-lysine
0.38
-
activity against L-glutamine
0.5
-
activity against L-isoleucine
0.67
-
activity against L-tyrosine
0.81
-
activity against L-glutaminic acid
1.16
-
after 389fold purification, using L-lysine as substrate, at pH 7.0 and 65°C
1.19
-
activity against L-histidine
1.56
-
crude venom
10
-
with L-glutamine as substrate
1020
untreated enzyme, expressed as 9His-LAAO4 in Pichia pastoris, pH 7, 30°C
110
purified enzyme, substrate L-Val
12.5
-
L-histidine
14.8
-
L-tyrosine
15
-
activity against L-leucine
15.5
-
L-tryptophan
16.6
-
L-arginine
17
-
L-isoleucine
17.4
-
S-adenosy-L-cysteine
18.4
-
L-asparagine
2
untreated enzyme, expressed as 6His-LAAO4 in Escherichia coli, pH 7, 30°C
20 - 40
acid-treated enzyme, expressed as 9His-LAAO4 in Pichia pastoris, as well as 6His-LAAO4 expressed in Escherichia coli, pH 7, 30°C
21
-
activity against L-methionine
21.4
-
L-ornithine
230
-
with L-alanine as substrate
24.3
-
L-methionine
25.2
-
S-adenosyl-L-homocysteine
26
-
purified native enzyme, pH 4.0, 37°C
3.15
-
purified enzyme
3.8
-
substrate L-isoleucine, pH 8.5, 25°C
30.5
-
L-methionine sulfoximine
300
-
substrate L-histidine, 25°C, pH 7.2
310
purified enzyme, substrate L-Trp
335
purified enzyme, substrate L-His
340
purified enzyme, substrate L-Arg
35
-
with L-serine as substrate
39.5
-
L-glutamine
4.4
-
L-threonine
4.6
homogenous enzyme, L-alanine as susbtrate, 30°C, pH 7.6
4.9
-
L-aspartate
40
-
with L-threonine or L-proline as substrate
410
-
substrate L-valine, 25°C, pH 7.2
430
-
with L-valine as substrate
440
-
substrate L-methionine, 25°C, pH 7.2
460
-
with L-methionine as substrate
487.7
-
12.9fold purified
5
-
with glycine or L-lysine as substrate
5.44
pH not specified in the publication, temperature not specified in the publication
5.5
-
substrate L-histidine, pH 8.5, 25°C
5.75
-
activity against L-arginine
545
purified enzyme, substrate L-Phe
575
purified enzyme, substrate L-Leu
6.04
purified recombinant enzyme, substrate L-phenylalanine, pH 8.5, 45°C
6.5
-
substrate L-leucine, pH 8.5, 25°C
6.94
-
isoform L2
60
purified enzyme, substrate L-Ala
635
-
with L-tyrosine as substrate
665
-
with L-phenylalanine as substrate
685
-
substrate L-tyrosine, 25°C, pH 7.2
7.2
-
substrate L-tryptophan, pH 8.5, 25°C
765
-
substrate L-phenylalanine, 25°C, pH 7.2
79.92
-
51.3fold purified
8.75
-
activity against L-tryptophan
8.96
-
isoform L1
800
-
substrate L-tyrosine, pH 8.5, 37°C
87.5
-
purified enzyme, substrate L-Leu
9
-
purified native enzyme, pH 4.7, 37°C
9.38
-
activity against L-phenylalanine
90
-
with L-arginine as substrate
additional information