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1.4.1.4: glutamate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about glutamate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.4.1.4

Reaction

L-glutamate
+
H2O
 +
NADP+
=
2-oxoglutarate
 +
NH3
 +
NADPH
 +
H+

Synonyms

APE1386, BpNADPGDH I, BpNADPGDH II, dehydrogenase, glutamate (nicotinamide adenine dinucleotide phosphate), EcGDH, GDH, GDH1, Gdh1p, GDH2, GDH3, Gdh3p, GDH4, GdhA, GDHB, GDHC, GDHI, GDHI', GDHII, GDHP, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamate dehydrogenase 3, glutamic acid dehydrogenase, glutamic dehydrogenase, GW612_14215, H-form specific BpNADPGDH II, hGDH, hyphal-form specific BpNADPGDH II, L-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NADP(+)-dependent glutamate dehydrogenase, NADP(H)-dependent glutamate dehydrogenase, NADP(H)-GDH, NADP(H)-specific glutamate dehydrogenase, NADP+ - dependant-glutamate dehydrogenase, NADP+-dependent GDH, NADP+-dependent glutamate dehydrogenase, NADP+-Gdh, NADP-dependent GDH, NADP-dependent GluDH, NADP-dependent glutamate dehydrogenase, NADP-dependent glutamate dehydrogenase 1, NADP-dependent glutamate dehydrogenase 2, NADP-GDH, NADP-GDH 1, NADP-GDH 2, NADP-glutamate dehydrogenase, NADP-linked glutamate dehydrogenase, NADP-specific glutamate dehydrogenase, NADP-specific L-glutamate dehydrogenase, NADP-ylGdh1p, NADPH-dependent GDH, NADPH-dependent glutamate dehydrogenase, OsGDH4, Pcal_1606, RocG, TrGDH, Y-form specific BpNADPGDH I, YALI0F17820g, yeast-form specific BpNADPGDH I, ylGDH1

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.4 glutamate dehydrogenase (NADP+)

Inhibitors

Inhibitors on EC 1.4.1.4 - glutamate dehydrogenase (NADP+)

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4-pyridinedicarboxylate
2-hydroxyethyl disulfide
attenuation of reductive amination (forward) activity but a negligible effect on oxidative deamination (reverse) activity
2-Methyleneglutarate
2-oxoglutarate
4-chloromercuribenzoate
Ag2+
-
at 1 mM, 60% inhibition
AgNO3
-
1 mM, 30°C, complete loss of aminating activity
AlCl3
BaCl2
-
1 mM, 30°C, 15% loss of aminating activity
benzene-1,3-dicarboxamide
-
-
bis(2,2,2-trifluoroethyl) benzene-1,3-dicarboxylate
-
-
CaCl2
-
1 mM, 30°C, 11% loss of aminating activity
CH2ICOOH
-
1 mM, 30°C, 15% loss of aminating activity
cystine
strongly and selectively inhibits the reductive amination reaction
dibenzyl benzene-1,3-dicarboxylate
-
-
dimethyl 4,4'-[1,3-phenylenebis(carbonylazanediyl)]di(cyclopent-2-ene-1-carboxylate)
-
-
dimethyl benzene-1,3-dicarboxylate
-
-
dimethyl ester of isophthalic acid
-
dimethyl ester of isophthalate (DMIP), but not isophthalate, inhibits Aspergillus niger growth on agar as well as in liquid culture. This is ascribed to the inability of isophthalate to enter fungal mycelia. Dimethyl ester of isophthalic acid is hydrolysed intracellularly to isophthalate. Subsequent to DMIP addition, intracellular isophthalate can be demonstrated. Addition of NH4+ to DMIP-treated Aspergillus niger mycelia results in intensive vacuolation, retraction of cytoplasm and autolysis
dipropan-2-yl benzene-1,3-dicarboxylate
-
-
dithiothreitol
-
1 mM, 30.7% residual activity
EDTA
-
1 mM, 5.3% residual activity
epigallocatechingallate
EGCG
-
FeSO3(NH4)2SO4
-
1 mM, 30°C, 14% loss of aminating activity
fumarate
glutamate
-
competitive inhibitor of the amination reaction
Glutaric acid
-
at 20 mM, 25% inhibition
glyoxylate
-
at 20 mM, 30% inhibition
guanidine hydrochloride
Hexachlorophene
15% inhibition at 0.016 mM; 80% inhibition at 0.016 mM
HgCl2
hydroxylamine
-
competitive inhibitor with ammonia and uncompetitive inhibitor with both 2-oxoglutarate and NADPH
iodoacetamide
-
at 4 mM, complete inactivation
isocitrate
10 M, 64% of initial activity
isophthalate
isophthalic acid
L-glutamate
-
substrate inhibition above 15 mM
L-Glutamic acid
-
at 20 mM 25% inhibition
L-homoserine
-
competitive inhibitor with respect to both ammonia and glutamine
L-tryptophan
-
at 20 mM, 15% inhibition
malate
MnCl2
N-ethylmaleimide
-
at 0.8 mM, 44% inhibition
N1,N1,N3,N3-tetra(propan-2-yl)benzene-1,3-dicarboxamide
-
-
N1,N3-bis(2-amino-2-oxoethyl)isophthalamide
-
-
N1,N3-bis(2-chlorophenyl)benzene-1,3-dicarboxamide
-
-
N1,N3-bis(3,5-dichlorophenyl)benzene-1,3-dicarboxamide
-
-
N1,N3-bis(3-fluorophenyl)benzene-1,3-dicarboxamide
-
-
N1,N3-bis(3-hydroxyphenyl)benzene-1,3-dicarboxamide
-
-
N1,N3-bis(4-fluorophenyl)benzene-1,3-dicarboxamide
-
-
N1,N3-bis(4-methoxyphenyl)benzene-1,3-dicarboxamide
-
-
N1,N3-bis(5-tert-butyl-2-hydroxyphenyl)benzene-1,3-dicarboxamide
-
-
N1,N3-bis[3,5-bis(trifluoromethyl)phenyl]benzene-1,3-dicarboxamide
-
-
N1,N3-bis[[3,5-bis(trifluoromethyl)phenyl]methyl]benzene-1,3-dicarboxamide
-
-
N1,N3-dihydroxybenzene-1,3-dicarboxamide
-
-
N1,N3-dimethoxybenzene-1,3-dicarboxamide
-
-
N1,N3-diphenylbenzene-1,3-dicarboxamide
-
-
NAD+
-
0.3 mM, 50.5% residual activity
NADH
-
0.3 mM, 5.3% residual activity
NADP+
-
inhibits at higher 2-oxoglutarate levels
NADPH
-
0.3 mM, 3.8% residual activity
nitrogen assimilation control protein
-
represses the transcription of the gene gdhA
-
O2
-
inactivation
oxaloacetate
p-chloromercuriphenyl sulfonate
-
inactivetes, can be reversed by addition of cysteine
p-hydroxymercuribenzoic acid
Pb(CH3COO)2
Pb2+
-
5 mM, 19.8% residual activity
potassium phosphate
-
over 0.1 M at oxidative deamination
propylselen
inhibits glutamate dehydrogenase by binding at the NADP+ binding site. No inhibition of enzyme mutant P320A
-
pyridoxal 5'-phosphate
pyruvate
-
at 10 mM slight inhibitory
sodium dodecylsulfate
-
at 0.7% w/v after 1 h 5% activity
succinate
-
competitive inhibitor with 2 oxoglutarate, uncompetitive with NADPH and non-competitive with ammonia
additional information
-