1.4.1.14: glutamate synthase (NADH)
This is an abbreviated version!
For detailed information about glutamate synthase (NADH), go to the full flat file.
Reaction
Synonyms
gltA, GltA2, gltB, glutamate (reduced nicotinamide adenine dinucleotide) synthase, glutamate synthase, glutamate synthase (NADH), glutamate synthase (NADH-dependent), glutamate synthetase, glutamine 2-oxoglutarate aminotransferase, GOGAT, GOGAT1, GOGAT2, L-glutamate synthase (NADH), L-glutamate synthetase, NADH glutamate synthase, NADH-dependent glutamate synthase, NADH-dependent glutamine-2-oxoglutarate aminotransferase, NADH-GltS, NADH-glutamate synthase, NADH-GOGAT, NADH-GOGAT I, NADH-GOGAT II, NADH-GOGAT1, NADH-GOGAT2, NADH: GOGAT, Os01g0681900, Os05g0555600, synthase, glutamate (reduced nicotinamide adenine dinucleotide)
ECTree
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Activating Compound
Activating Compound on EC 1.4.1.14 - glutamate synthase (NADH)
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2-mercaptoethanol
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1%, presence of a reducing agent is essential for enzyme activity
diphosphate
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5 mM, stimulates activity with 36% enhancement
dithiothreitol
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0.5 mg per ml, presence of a reducing agent is essential for enzyme activity
GltB
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GltA and not GltA2 is dependent on GltB for GOGAT activity in vivo
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NH4Cl
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NADH-GOGAT gene expression is transiently induced within a few hours of supplying either 1 or 25 mM to the medium, leading to a marked accumulation of NADH-GOGAT mRNA
NH4NO3
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transcripts for NADH-GOGAT transiently accumulate when 0.5 mM is applied
succinate
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50 mM, stimulates activity by 57%
additional information
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not activated by pyridoxal 5-phosphate and pyridoxamine 5-phosphate
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additional information
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supply of NH4+ at low concentrations increases the expression of the enzyme: supply with 1 mM NH4+ for 24 h increases the enzyme content in the epidermis and exodermis of the root surface
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additional information
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supply of NH4+ at low concentrations increases the expression of the enzyme: supply with 1 mM NH4+ for 24 h increases the enzyme content in the epidermis and exodermis of the root surface
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additional information
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NH4+ transiently induces accumulation of enzyme mRNA in roots of rice seedlings, maximally 3-6 h after supply
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