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1.3.7.13: 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin)

This is an abbreviated version!
For detailed information about 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin), go to the full flat file.

Word Map on EC 1.3.7.13

Reaction

protochlorophyllide a
+ 2 oxidized ferredoxin [iron-sulfur] cluster =
3,8-divinyl protochlorophyllide a
 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+

Synonyms

3,8-divinyl chlorophyllide reductase, 8-vinyl reductase, 8V reductase, 8VR, AM1_2849, bciB, C8 vinyl reductase, C8-vinyl reductase, C8-vinyl-reductase, chlorophyllide a oxidoreductase, Ctha_1208, cvrA, cyano-type divinyl chlorophyllide a 8-vinyl-reductase, DVR, F-DVR, Fd-dependent DVR, ferredoxin-dependent 8-vinyl-protochlorophyllide reductase, frhB, nucleoside-diphosphate-sugar epimerase, PChlide reductase, RSP_3070, Slr1923, Slr1923 potein, SyF-DVR

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.7 With an iron-sulfur protein as acceptor
                1.3.7.13 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin)

Cloned

Cloned on EC 1.3.7.13 - 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803
-
expressed in Escherichia coli
-
gene AM1_2849, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli
gene bciB or frhB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, frhB is introduced into an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803, and restores synthesis of the pigment with an ethyl group at C-8, demonstrating its activity as 8-vinyl reductase, subcloning in Escherichia coli strain JM109
gene bciB, cloning in Escherichia coli strain JM109, recombinant expression of C-terminally His6-tagged in Escherichia coli strain BL21(DE3). Plasmid pET28-CthaBciB is cotransformed into strain BL21(DE3) along with the plasmid pDB1282, which contains the genes iscS, iscU, iscA, hscA, hscB, and fdx for Fe/S-cluster assembly from Azotobacter vinelandii under the control of an arabinose-inducible promoter to produce Escherichia coli strain AHS-1
gene slr1923, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli
gene slr1923, phylogenetic analysis