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1.3.3.5: bilirubin oxidase

This is an abbreviated version!
For detailed information about bilirubin oxidase, go to the full flat file.

Word Map on EC 1.3.3.5

Reaction

2 bilirubin +

O2
= 2 biliverdin + 2 H2O

Synonyms

bilirubin oxidase, bilirubin oxidase M-1, bilirubin:oxygen oxidoreductase, blue Cu enzyme, BOD, BODx, BOX, BPUM_0542, copper oxidase, CotA, MCO, multicopper oxidase, MvBO, MvBOD, oxidase, bilirubin

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.5 bilirubin oxidase

Purification

Purification on EC 1.3.3.5 - bilirubin oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex-G-100 gel filtration
-
ammonium sulfate, charcoal, Sephadex a-50, Sephadex G-100
-
by gel filtration
DEAE-Sepharose Fast Flow column chromatography and Sephadex G-100 gel filtration
-
extracellular enzyme 3.92fold by ammonium sulfate fractionation and anion exchange chromatography, an additional step of gel filtration lowers the enzyme purity and amount
-
native enzyme by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
native enzyme by anion exchange chromatography and hydrophobic interaction chromatography
-
Ni-NTA column chromatography
Q sepharose column chromatography
recombinant enzyme
-
recombinant His-tagged enzyme from Escherichia coli strain Origami B (DE3) by nickel affinity chromatography and dialysis
recombinant holo-enzyme from Pichia pastoris strain GS115 to homogeneity by hydrophobic interaction chromatography
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recombinant wild-type enzyme and mutants
-
the commercial preparation of bilirubin oxidase from Myrothecium verrucaria is additionally purified by anion exchange chromatography
to homogeneity
Toyopearl Butyl-650M column chromatography and Superdex 200 gel filtration
-