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1.3.3.3: coproporphyrinogen oxidase

This is an abbreviated version!
For detailed information about coproporphyrinogen oxidase, go to the full flat file.

Word Map on EC 1.3.3.3

Reaction

Coproporphyrinogen III
+
O2
 + 2 H+ =
protoporphyrinogen-IX
 + 2 CO2 + 2 H2O

Synonyms

copro'gen oxidase, Coprogen oxidase, coproporphyinogen oxidase, coproporphyrinogen III oxidase, coproporphyrinogen oxidase, coproporphyrinogen-III oxidase, coproporphyrinogenase, COX, CPgen oxidase, CPGox, CPO, CPO III oxidase, CPOX, CPOX4, CPX, CPX1, CPX2, HEM13, Hem13p, HemF, HEMN1, KlHEM13, LIN2, LMM2, O2-dependent coproporphyrinogen III oxidase, oxygen-dependent coproporphyrinogen III oxidase, oxygen-dependent coproporphyrinogen-III oxidase, Sll1185

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.3 coproporphyrinogen oxidase

Purification

Purification on EC 1.3.3.3 - coproporphyrinogen oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
69fold purificationcalcium phosphate gel
-
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Ultrogel Ac44 gel filtration
by Ni2+ affinity chromatography
by nickel affinity chromatography
-
Ni-NTA agarose metal affinity resin chromatography
Ni2+ affinity chromatography
-
Ni2+ affinity column chromatography
-
nickel affinity column chromatography
-
partial
recombinant enzyme from Escherichia coli, 3-step procedure, 119fold to homogeneity
-
recombinant HA-tagged wild-type enzyme and His-tagged mutant enzyme from Escherichia coli by nickel and heparin affinity chromatography, respectively
recombinant hemF oxygen-dependent enzyme form to homogeneity by affinity chromatography, the tag protein is cleaved off by thrombin, recombinant wild-type and mutant enzymes
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli, cleavage of the tag
-
recombinant protein, Ni-NTA agarose metal affinity chromatography
recombinant terminally Strep-tagged HemF from Escherichia coli to homogeneity
separation of coproporphyrinogen oxidase, EC 1.3.3.3, from uroporphyrinogen decarboxylase, EC 4.1.1.37, by coproporphyrinogen-III-resin affinity chromatography, elution in a salt gradient
-
separation of uroporphyrinogen decarboxylase, EC 4.1.1.37, from coproporphyrinogen oxidase using porphyrin-affinity chromatography
-
TALON metal affinity resin column chromatography
to homogeneity, chromatography steps
to homogeneity, chromatography techniques
-
to homogeneity, recombinant enzyme