1.3.3.12: L-galactonolactone oxidase
This is an abbreviated version!
For detailed information about L-galactonolactone oxidase, go to the full flat file.
Word Map on EC 1.3.3.12
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1.3.3.12
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flavin
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non-covalently
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cruzi
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l-gulonolactone
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chagas
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l-ascorbate
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n-ethylmaleimide
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iodoacetamide
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trypanosomal
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pharmacology
- 1.3.3.12
- flavin
-
non-covalently
- cruzi
- l-gulonolactone
-
chagas
- l-ascorbate
- n-ethylmaleimide
- iodoacetamide
-
trypanosomal
- pharmacology
Reaction
Synonyms
EC 1.1.3.24, GAL, galactone-gamma-lactone oxidase, galactonolactone oxidase, galactonolactone oxidoreductase, GL oxidase, L-galactono-1,4-lactone oxidase, L-galactono-gamma-lactone oxidase
ECTree
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Engineering
Engineering on EC 1.3.3.12 - L-galactonolactone oxidase
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A113G
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site-directed mutagenesis, in the reaction with 1,4-benzoquinone as electron acceptor, the activity of the dimeric formof A113G is about 1.5fold higher compared to the monomer, the specific activity of the A113G variant in the reaction with molecular oxygen is about 2.5-3times lower than with 1,4-benzoquinone
K450G
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the substitution results in an enzyme that retains an ability to bind FMN (around 50%of the wild type enzyme), but which exhibits no activity
K55G
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the mutation has only a minor inhibitory effect on both FMN binding and biochemical activity
K55H
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the mutation causes dramatically reduced FMN binding and enzyme activity (above 90%)
A113G
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site-directed mutagenesis, in the reaction with 1,4-benzoquinone as electron acceptor, the activity of the dimeric formof A113G is about 1.5fold higher compared to the monomer, the specific activity of the A113G variant in the reaction with molecular oxygen is about 2.5-3times lower than with 1,4-benzoquinone
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