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1.3.3.11: pyrroloquinoline-quinone synthase

This is an abbreviated version!
For detailed information about pyrroloquinoline-quinone synthase, go to the full flat file.

Word Map on EC 1.3.3.11

Reaction

6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate
+ 3 O2 =
4,5-dioxo-4,5-dihydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate
 + 2 H2O2 + 2 H2O

Synonyms

PqqC, PqqC/D, pyrroloquinoline quinone synthase C, pyrroloquinoline-quinone synthase, pyrroloquinoline/quinone synthesis protein C, quinoprotein dehydrogenase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.11 pyrroloquinoline-quinone synthase

Crystallization

Crystallization on EC 1.3.3.11 - pyrroloquinoline-quinone synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme mutants H154S/PQQ Y175F/PQQ Y175S/R179S in complex with intermediate 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid, sitting drop-vapor diffusion method, optimized conditions for each mutant: 0.1 M HEPES, pH 7.0, 0.5% w/v PEG 8000 for mutant H154S/PQQ complex, 0.2 M ammonium sulfate, 0.1 M Bis-Tris, pH 6.5, 25% w/v PEGl 3350 for mutant Y175F/PQQ complex, and 0.2 M sodium chloride, 0.1 M Tris, pH 8.5, 25% w/v PEG 3350 for mutant R179S/Y175S/intermediate complex, all at 20°C, X-ray diffraction structure determination and analysis at 1.3-2.35 A resolution
sitting drop vapor diffusion method