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1.3.1.B13: polyprenol reductase (NADPH)

This is an abbreviated version!
For detailed information about polyprenol reductase (NADPH), go to the full flat file.

Word Map on EC 1.3.1.B13

Reaction

a dolichol
+
NADP+
=
a polyprenol
+
NADPH
+
H+

Synonyms

polyprenol reductase, polyprenol reductase 1, polyprenol reductase 2, polyprenol reductase2, PPRD1, PPRD2, SRD5A3

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.B13 polyprenol reductase (NADPH)

Cloned

Cloned on EC 1.3.1.B13 - polyprenol reductase (NADPH)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in HEK293T cells
recombinant expression of isozyme PPRD1 in Saccharomyces cerevisiae strains BY4741, L5366, and mutant strains dfg10DELTA and dfg10-100 from lasmid pYES-DEST52, recombinant expression of isozyme PPRD1 in Escherichia coli strain BL21, method optimization. Agrobacterium-mediated transient coexpression of GFP-tagged isozyme with plant organelle markers (mCherry fusions) in Nicotiana benthamiana. Quantitative PCR enzyme expression and promoter activity analysis
recombinant expression of isozyme PPRD1 in Saccharomyces cerevisiae strains BY4741, L5366, and mutant strains dfg10DELTA and dfg10-100 from lasmid pYES-DEST52, recombinant expression of wild-type an dmutant isozyme PPRD1s in Escherichia coli strain BL21, method optimization. Agrobacterium-mediated transient coexpression of GFP-tagged isozyme with plant organelle markers (mCherry fusions) in Nicotiana benthamiana. Quantitative PCR enzyme expression and promoter activity analysis