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1.3.1.98: UDP-N-acetylmuramate dehydrogenase

This is an abbreviated version!
For detailed information about UDP-N-acetylmuramate dehydrogenase, go to the full flat file.

Word Map on EC 1.3.1.98

Reaction

UDP-N-acetyl-alpha-D-muramate
+
NADP+
=
UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
+
NADPH
+
H+

Synonyms

alr5066, EC 1.1.1.158, More, MurB, MurBAb, PA2977, PaMurB, reductase, uridine diphosphoacetylpyruvoylglucosamine, type I UNAGEP reductase, UDP-GlcNAc-enoylpyruvate reductase, UDP-N-acetylenolpyruvoylglucosamine reductase, UDP-N-acetylenolpyruvyl glucosamine reductase, UDP-N-acetylenolpyruvylglucosamine reductase, UDP-N-acetylglucosamine-enoylpyruvate reductase, UDP-N-acetylmuramate dehydrogenase, UNAGEP reductase, uridine diphospho-N-acetylglucosamine-enolpyruvate reductase, uridine-5'-diphospho-N-acetyl-2-amino-2-deoxy-3-O-lactylglucose:NADP-oxidoreductase, VspiD_010100018130

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.98 UDP-N-acetylmuramate dehydrogenase

Purification

Purification on EC 1.3.1.98 - UDP-N-acetylmuramate dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Superdex 200 gel filtration
-
Ni-NTA agarose column chromatography
-
recombinant enzyme from strain BL21(DE3) by ammonium sulfate fractionation, hydrophobic interaction and ion exchange chromatography to homogeneity
-
recombinant His-tagged enzyme from Escherichia coli K12S by nickel affinity chromatography
-
recombinant MurB from Escherichia coli strain BL21(DE3)
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, followed by a second step of affinity chromatography to remove uncleaved protein and the His6-tagged TEV protease, further purification by preparation scale gel filtration, and ultrafiltration
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) to homogeneity, native wild-type enzyme from Staphylococcus aureus 10fold by ammonium sulfate precipitation and two steps of anion exchange chromatography