1.3.1.95: acrylyl-CoA reductase (NADH)
This is an abbreviated version!
For detailed information about acrylyl-CoA reductase (NADH), go to the full flat file.
Word Map on EC 1.3.1.95
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1.3.1.95
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dehydratase
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acrylate
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3-hydroxypropionate/4-hydroxybutyrate
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sedula
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3-hydroxypropionate
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3-hydroxypropionyl-coa
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metallosphaera
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ferredoxin
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propionyl-coa
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fmn
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aurantiacus
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dmdas
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dimethylsulfoniopropionate
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ketone
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propionicum
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vinyl
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succinyl-coa
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lyases
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demethylase
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semialdehyde
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acuis
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roseobacters
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chloroflexus
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sulfide
- 1.3.1.95
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dehydratase
- acrylate
-
3-hydroxypropionate/4-hydroxybutyrate
- sedula
- 3-hydroxypropionate
- 3-hydroxypropionyl-coa
- metallosphaera
-
ferredoxin
- propionyl-coa
- fmn
- aurantiacus
-
dmdas
- dimethylsulfoniopropionate
- ketone
- propionicum
-
vinyl
- succinyl-coa
-
lyases
- demethylase
-
semialdehyde
-
acuis
- roseobacters
- chloroflexus
- sulfide
Reaction
Synonyms
Acr, acryloyl-CoA reductase, acryloyl-CoA reductase (NADPH), acryloyl-coenzyme A reductase
ECTree
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Substrates Products
Substrates Products on EC 1.3.1.95 - acrylyl-CoA reductase (NADH)
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REACTION DIAGRAM
(E)-2-butenoate + methyl viologen
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less than 2% activity of that with ethyl vinyl ketone
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-
?
(E)-2-butenoyl-CoA + methyl viologen
?
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second best substrate, 45% activity compared to acryloyl-CoA
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-
?
1-penten-3-one + methyl viologen
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less than 2% activity of that with ethyl vinyl ketone
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-
?
2-cyclohexen-1-one + methyl viologen
?
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less than 2% activity of that with ethyl vinyl ketone
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-
?
2-methylacrolein + methyl viologen
?
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less than 2% activity of that with ethyl vinyl ketone
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-
?
4-phenyl-3-buten-2-one + methyl viologen
?
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less than 2% activity of that with ethyl vinyl ketone
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-
?
acrylic acid + methyl viologen
?
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less than 2% activity of that with ethyl vinyl ketone
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-
?
butyryl-CoA + 2,6-dichlorophenolindophenol
?
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about 1% of the reduction rate of (E)-2-butenoyl-CoA and methyl viologen
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-
?
diethyl ketone + methyl viologen
?
-
less than 2% activity of that with ethyl vinyl ketone
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-
?
ethyl acrylate + methyl viologen
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less than 2% activity of that with ethyl vinyl ketone
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-
?
methyl propenyl ketone + methyl viologen
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less than 2% activity of that with ethyl vinyl ketone
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-
?
methyl vinyl ketone + methyl viologen
?
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less than 2% activity of that with ethyl vinyl ketone
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-
?
acryloyl-CoA + NADH + H+
propanoyl-CoA + NAD+
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-
-
?
acryloyl-CoA + NADPH + H+
propanoyl-CoA + NADP+
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100% specificity
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-
ir
?
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the enzyme complex also exhibits acyl-CoA dehydrogenase activity with propionyl-CoA or butyryl-CoA as electron donor and ferricenium hexafluorophosphate as acceptor. The enzyme also catalyses the oxidation of NADH by iodonitrosotetrazolium chloride (diaphorase activity) or by air, which leads to the formation of H2O2 (NADH oxidase activity)
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-
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additional information
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the enzyme shows no activity with different concentrations of NAD(P)H in 0.1 M phosphate buffer pH 7.0 and ethyl vinyl ketone as substrate
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-
?
additional information
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less than 1% specificity for crotonyl-CoA
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-
?