1.3.1.93: very-long-chain enoyl-CoA reductase
This is an abbreviated version!
For detailed information about very-long-chain enoyl-CoA reductase, go to the full flat file.
Word Map on EC 1.3.1.93
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1.3.1.93
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cuticular
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vlcfas
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sphingolipids
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acyl-coas
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triacylglycerols
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piecemeal
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taxus
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antidiabetic
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eceriferum
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sumatrana
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alkane
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invaginations
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endophytic
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nucleus-vacuole
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cuticle
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colletotrichum
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elongase
- 1.3.1.93
- cuticular
-
vlcfas
- sphingolipids
- acyl-coas
- triacylglycerols
-
piecemeal
- taxus
-
antidiabetic
-
eceriferum
- sumatrana
- alkane
-
invaginations
-
endophytic
-
nucleus-vacuole
- cuticle
- colletotrichum
- elongase
Reaction
Synonyms
At3g55360, CER10, EC 2.3.1.119, ECR, ECR1, ECR2, enoyl reductase, TER, trans-2-enoyl-CoA reductase, TSC13, very-long-chain enoyl-CoA reductase
ECTree
Advanced search results
Engineering
Engineering on EC 1.3.1.93 - very-long-chain enoyl-CoA reductase
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G225A
mutant is not able to complement the growth defect of a yeast tsc13 mutant
G234A
mutant is not able to complement the growth defect of a yeast tsc13 mutant
I231A
mutant is not able to complement the growth defect of a yeast tsc13 mutant
P232A
mutant is not able to complement the growth defect of a yeast tsc13 mutant
D77A
mutation is not critical for function, mutant is able to complement an enzyme deletion mutant
E144A
mutation is not critical for function, mutant is able to complement an enzyme deletion mutant
E259A
mutation is not critical for function, mutant is able to complement an enzyme deletion mutant
E91A
mutation is not critical for function, mutant is able to complement an enzyme deletion mutant
H137A
mutation is not critical for function, mutant is able to complement an enzyme deletion mutant
H149A
mutation is not critical for function, mutant is able to complement an enzyme deletion mutant
K140A
mutant is not able to complement an enzyme deletion mutant. Mutant protein is present at wild-type levels and does not show altered membrane topology
K76A
mutation is not critical for function, mutant is able to complement an enzyme deletion mutant
R141A
mutant is not able to complement an enzyme deletion mutant. Mutant protein is present at wild-type levels and does not show altered membrane topology
Y103A
mutation is not critical for function, mutant is able to complement an enzyme deletion mutant
Y138A
mutant is not able to complement an enzyme deletion mutant, protein is unstable
additional information
HeLa cells are transfected with control siRNA or TER si RNA, siRNA-generated enzyme knockout mutant. Knockdown of TER in HeLa cells causes decreased sphingosine 1-phosphate metabolism in vitro and a reduction in the dihydrosphingosine metabolism
additional information
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with the aim of commercially interesting production of phenylpropanoids, such as flavonoids and stilbenoids, site saturation mutagenesis is used for modification of the enzyme to reduce the side activity without disrupting the natural function, identification of a number of amino acid changes which slightly increase flavonoid production but without reducing the formation of side product. The complementation of TSC13 by the gene homologue from plants essentially eliminates the unwanted side reaction, while retaining the productivity of phenylpropanoids in a simulated fed batch fermentation. The native open reading frame (ORF) of TSC13 is replaced by gene homologues from Arabidospis thaliana (AtECR), Gossypium hirsutum (GhECR2) and Malus domestica (MdECR), using a split URA3 cassette under the control of the native TSC13 promoter
additional information
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YRF50 cells (BY4741,pTSC13::KanMX4-tTA-ptetO7) are constructed by replacing the promoter of the TSC13 gene (pTSC13) with tetO7 promoter (ptetO7) using the KanMX4-tTA-ptetO7 cassette from the pCM225 plasmid. Strains ABY83 and ABY80 cells are constructed by deletion of the TSC13 gene in BY4741 cells bearing the pTW6 or pAB119 plasmid, respectively, using a tsc13DELTA::LEU2 fragment by homologous recombination. Generation of Saccharomyces cerevisiae Tsc13-lowered cells
additional information
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YRF50 cells (BY4741,pTSC13::KanMX4-tTA-ptetO7) are constructed by replacing the promoter of the TSC13 gene (pTSC13) with tetO7 promoter (ptetO7) using the KanMX4-tTA-ptetO7 cassette from the pCM225 plasmid. Strains ABY83 and ABY80 cells are constructed by deletion of the TSC13 gene in BY4741 cells bearing the pTW6 or pAB119 plasmid, respectively, using a tsc13DELTA::LEU2 fragment by homologous recombination. Generation of Saccharomyces cerevisiae Tsc13-lowered cells
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