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1.3.1.91: tRNA-dihydrouridine20 synthase [NAD(P)+]

This is an abbreviated version!
For detailed information about tRNA-dihydrouridine20 synthase [NAD(P)+], go to the full flat file.

Word Map on EC 1.3.1.91

Reaction

5,6-dihydrouracil20 in tRNA
+
NAD(P)+
=
uracil20 in tRNA
 +
NAD(P)H
 +
H+

Synonyms

dihydrouridine synthase, dihydrouridine synthase 2, dihydrouridine synthase C, Dus, DUS 2, DUS2, Dus2p, DusA, DusB, DusC, EcoDusC, hDUS2, HsDus2, SMM1, tRNA-dihydrouridine synthase 2, tRNA-dihydrouridine synthases

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.91 tRNA-dihydrouridine20 synthase [NAD(P)+]

Purification

Purification on EC 1.3.1.91 - tRNA-dihydrouridine20 synthase [NAD(P)+]

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HiTrap column chromatography and Superdex S200 gel filtration
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni-NTA column chromatography and Superdex S200 gel filtration
recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli cell-free extract (centrifugation at 40000 x g) by nickel affinity chromatography, dialysis, heparin affinity chromatography, and gel filtration
recombinant wild-type and selenomethionine-labeled hDus2 1-340 fragment, comprising the hDus2 catalytic and tRNA-recognition domains, from Escherichia coli strains BL21(DE3) and B834(DE3), respectively