1.3.1.84: acrylyl-CoA reductase (NADPH)

This is an abbreviated version!
For detailed information about acrylyl-CoA reductase (NADPH), go to the full flat file.

Word Map on EC 1.3.1.84

1.3.1.84

propionyl-coa

acrylate

acuis

3-hydroxypropionate

3-hydroxypropionyl-coa

dehydratase

chloroflexus

sedula

crotonyl-coa

aurantiacus

ruegeria

dimethylsulfoniopropionate

metallosphaera

3-hydroxypropionate/4-hydroxybutyrate

hyper-sensitive

pomeroyi

lyases

dmdas

ethylmalonyl-coa

sphaeroides

roseobacters

succinyl-coa

synthesis

Reaction

Synonyms

Acr, acryloyl-CoA reductase, acryloyl-CoA reductase (NADPH), acryloyl-coenzyme A reductase, acrylyl-CoA reductase, acrylyl-coenzyme A reductase, AcuI, Msed_1426, NADPH-dependent acryloyl-CoA reductase activity, NADPH-dependent acrylyl-CoA reductase, RSP_1434, SPO1914, SPO_1914, yhdH

ECTree

1 Oxidoreductases

1.3 Acting on the CH-CH group of donors

1.3.1 With NAD⁺ or NADP⁺ as acceptor

1.3.1.84 acrylyl-CoA reductase (NADPH)

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
6	Activating Compound
568	AA Sequence
2	Application
1	CAS Registry Number
8	Cloned(Commentary)
17	Cofactor
2	Expression
18	General Information
1	Inhibitors
7	kcat/KM [mM/s]
17	KM Value [mM]
1	Localization
9	Metals/Ions
13	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
16	Organism
9	Pathway
4	PDB ID
6	pH Optimum
1	pH Range
6	Protein Variants
8	Purification (Commentary)
3	Reaction
14	Reference
3	Source Tissue
13	Specific Activity [micromol/min/mg]
1	Storage Stability
34	Substrates and Products (Substrate)
13	Subunits
45	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
7	Turnover Number [1/s]

Purification

print visible entries

print all entries

Purification on EC 1.3.1.84 - acrylyl-CoA reductase (NADPH)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Purification (commentary) Search

native enzyme by anion exchange and hydrophobic interaction chromatography, ultrafiltration, hydroxyapatatite chromatography, and again ultrafiltration. The enzyme is separated from acryloyl-CoA hydratase

Ruegeria pomeroyi

Q5LS56

745800

Ni-NTA column chromatography and Superose 12 gel filtration

3 entries

recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2(DE3) by immobilized metal affinity chromatography and gel filtration

Metallosphaera sedula

A4YGN2

745689

recombinant His10-tagged enzyme 11fold from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration

Cereibacter sphaeroides

Q3J6K9

745213

recombinant His10-tagged enzyme 4fold from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration

Ruegeria pomeroyi

Q5LS56

745213

recombinant His10-tagged enzyme 6fold from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration

Escherichia coli

P26646

745213