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1.3.1.32: maleylacetate reductase

This is an abbreviated version!
For detailed information about maleylacetate reductase, go to the full flat file.

Word Map on EC 1.3.1.32

Reaction

3-oxoadipate
+
NAD(P)+
=
2-maleylacetate
+
NAD(P)H
+
H+

Synonyms

GraC, hadD, hqdD, HqoD, HxqD, MAA reductase, maleoylacetate reductase, maleylacetate reductase, MAR, PcpE, pdcF, PnpD, TcpD, tfdF, tsdD

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.32 maleylacetate reductase

Crystallization

Crystallization on EC 1.3.1.32 - maleylacetate reductase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His-tagged wild-type and SeMet-labeled PnpD, the sitting-drop vapour-diffusion method, 0.002 ml of 15 mg/ml protein in 20 mM Tris, pH 8.0, 5 mM NADH, and 20 mM NaCl are mixed with 0.002 ml of precipitant solution containing 0.2 M ammonium phosphate dibasic and 20% w/v PEG 3350, equilibration against 0.2 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 2.9 A resolution
sitting drop vapor diffusion method, using 1.1-1.4 M ammonium sulfate, 0.1 M sodium chloride, 2% (w/v) benzamidine-HCl and 0.1 M Na HEPES pH 7.5 at 20°C
sitting drop vapour diffusion method, using ammonium sulfate as the precipitating agent, at 20°C
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