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1.3.1.32: maleylacetate reductase

This is an abbreviated version!
For detailed information about maleylacetate reductase, go to the full flat file.

Word Map on EC 1.3.1.32

Reaction

3-oxoadipate
+
NAD(P)+
=
2-maleylacetate
 +
NAD(P)H
 +
H+

Synonyms

GraC, hadD, hqdD, HqoD, HxqD, MAA reductase, maleoylacetate reductase, maleylacetate reductase, MAR, PcpE, pdcF, PnpD, TcpD, tfdF, tsdD

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.32 maleylacetate reductase

Cloned

Cloned on EC 1.3.1.32 - maleylacetate reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned and expressed in Escherichia coli
-
cloning of the clcE gene encoding maleylacetate reductase from Pseudomonas sp. strain B13
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
-
gen tftE cloned and overexpressed in Escherichia coli
-
gene macA coding for one of two maleylacetate reductases cloned
-
gene macA, DNA sequence determination and analysis, gene cluster analysis, the cluster also comprises genes macB and macR, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, overexpression in Escherichia coli strain BL21(DE3)
gene pnpD, expression of N-terminally His-tagged wild-type and SeMet-labeled PnpD in Escherichia coli BL21 (DE3)
into a pT7Blue T-Vector and expressed in Escherichia coli BL21 Star (DE3)
maleylacetate reductase gene cloned and sequenced
-
maleylacetate reductase genes from Alcaligenes eutrophus JMP289 and 335 cloned
-
overexpression in Escherichia coli BL21-AI cells
the chromosome of the nonfluorescent Pseudomonas sp. strain PKO1 cloned using plasmid pRO1727, the 0.5 kb BamH1 DNA fragment contains the gene for maleylacetate reductase, cloned mar gene is carried in cells of Pseudomonas aeruginosa and Pseudomonas putida
-
the hqoD gene is cloned downstream of the arabinose-inducible promoter PBAD in the pBS1 vector to generate pBS1hqoD plasmids and transferred to Pseudomonas putida KT2442
-
the hxqD gene is cloned downstream of the arabinose-inducible promoter PBAD in the pBS1 vector to generate pBS1hxqD plasmids and transferred to Pseudomonas putida KT2442
-
the tcpD gene is cloned downstream of the arabinose-inducible promoter PBAD in the pBS1 vector to generate pBS1tcpD, plasmids and transferred to Pseudomonas putida KT2442
-