1.3.1.3: DELTA4-3-oxosteroid 5beta-reductase This is an abbreviated version! For detailed information about DELTA4-3-oxosteroid 5beta-reductase, go to the full flat file .
Reaction
17,21-dihydroxy-5beta-pregnane-3,11,20-trione +
NADP+ =
cortisone +
NADPH +
H+
Synonyms 3-oxo-DELTA4-steroid 5beta-reductase, 4,5beta-dihydrocortisone:NADP+ DELTA4-oxidoreductase, 5-beta-reductase, 5beta-POR, 5beta-red, AKR1D1, AKR1D4, aldo-keto reductase, aldo-keto reductase 1D1, aldo–keto reductase 1D1, androstenedione 5beta-reductase, CARUB_v10005016mg, cortisone 5beta-reductase, cortisone beta-reductase, DELTA 4-5beta-reductase, DELTA4-3-ketosteroid 5beta-reductase, DELTA4-3-oxosteroid 5beta-reductase, DELTA4-hydrogenase, EC 1.3.1.23, EC 1.3.1.3, h5beta-red, h5beta-reductase, More, P5betaR, progesterone 5beta-reductase, reductase, cholestenone 5beta.-, reductase, cortisone DELTA4-5beta-, SRD5beta, St5betaR1, steroid 5beta-reductase, steroid 5beta.-reductase, testosterone 5-beta-reductase, testosterone 5beta-reductase
ECTree
Cloned
Cloned on EC 1.3.1.3 - DELTA4-3-oxosteroid 5beta-reductase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutant enzymes in Escherichia coli strain M15
expressed in Escherichia coli
expressed in Escherichia coli strain C41(DE3)
-
expressed in Escherichia coli strain M15
expression in Escherichia coli
expression in Escherichia coli and HEK-293 cell
expression in human HEK-293 cells
-
expression of GST-tagged enzyme in Escherichia coli
expression of splice variant AKR1D1 in HEK-293 cell
full-length P5betaR cDNA overexpressed in Escherichia coli as a fusion protein with MBP or His-tag
-
gene SRD5B1, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes
overproduced by the pQE expression system and transformed into Escherichia coli strain B834(DE3)
-
recombinant expression of AKR1D1 enzyme mutants in HEK-293 cells
recombinant expression of AKR1D1 enzyme mutants in HEK-293 cells only at low levels possible and with low activities
-
Sph1/Sal1 5beta-POR fragment cloned into the pQE vector and transformed into Escherichia coli strain M15(pREP4)
SPHI /SALI IC5beta-POR fragment cloned into the pQE vector system and transformed into Escherichai coli strain M15(pREP4)
DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutant enzymes in Escherichia coli strain M15
-
DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutant enzymes in Escherichia coli strain M15
expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
-