Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.3.1.3: DELTA4-3-oxosteroid 5beta-reductase

This is an abbreviated version!
For detailed information about DELTA4-3-oxosteroid 5beta-reductase, go to the full flat file.

Word Map on EC 1.3.1.3

Reaction

17,21-dihydroxy-5beta-pregnane-3,11,20-trione
+
NADP+
=
cortisone
+
NADPH
+
H+

Synonyms

3-oxo-DELTA4-steroid 5beta-reductase, 4,5beta-dihydrocortisone:NADP+ DELTA4-oxidoreductase, 5-beta-reductase, 5beta-POR, 5beta-red, AKR1D1, AKR1D4, aldo-keto reductase, aldo-keto reductase 1D1, aldo–keto reductase 1D1, androstenedione 5beta-reductase, CARUB_v10005016mg, cortisone 5beta-reductase, cortisone beta-reductase, DELTA 4-5beta-reductase, DELTA4-3-ketosteroid 5beta-reductase, DELTA4-3-oxosteroid 5beta-reductase, DELTA4-hydrogenase, EC 1.3.1.23, EC 1.3.1.3, h5beta-red, h5beta-reductase, More, P5betaR, progesterone 5beta-reductase, reductase, cholestenone 5beta.-, reductase, cortisone DELTA4-5beta-, SRD5beta, St5betaR1, steroid 5beta-reductase, steroid 5beta.-reductase, testosterone 5-beta-reductase, testosterone 5beta-reductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.3 DELTA4-3-oxosteroid 5beta-reductase

Cloned

Cloned on EC 1.3.1.3 - DELTA4-3-oxosteroid 5beta-reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutant enzymes in Escherichia coli strain M15
expressed in Escherichia coli
expressed in Escherichia coli strain C41(DE3)
-
expressed in Escherichia coli strain M15
expression in Escherichia coli
expression in Escherichia coli and HEK-293 cell
expression in human HEK-293 cells
-
expression of GST-tagged enzyme in Escherichia coli
expression of splice variant AKR1D1 in HEK-293 cell
full-length P5betaR cDNA overexpressed in Escherichia coli as a fusion protein with MBP or His-tag
-
gene SRD5B1, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes
overproduced by the pQE expression system and transformed into Escherichia coli strain B834(DE3)
-
pseudogene found
-
recombinant expression of AKR1D1 enzyme mutants in HEK-293 cells
recombinant expression of AKR1D1 enzyme mutants in HEK-293 cells only at low levels possible and with low activities
-
Sph1/Sal1 5beta-POR fragment cloned into the pQE vector and transformed into Escherichia coli strain M15(pREP4)
SPHI /SALI IC5beta-POR fragment cloned into the pQE vector system and transformed into Escherichai coli strain M15(pREP4)