1.3.1.12: prephenate dehydrogenase

This is an abbreviated version!
For detailed information about prephenate dehydrogenase, go to the full flat file.

Reaction

Synonyms

AceF, AroQ, bifunctional T-protein, chorismate mutase-prephenate dehydratase, chorismate mutase-prephenate dehydrogenase bifunctional enzyme, chorismate mutase-T:prephenate dehydrogenase bifunctional enzyme, Chorismate mutase/prephenate dehydratase, CM-PD, CM-TyrAp, CM/PDT/PDHG, CMPD, dehydrogenase, prephenate, hydroxyphenylpyruvate synthase, PD, PDH, pdhE-1, PDHG, T-protein, TYR1, tyrA, TyrA dehydrogenase, TyrAp

ECTree

     1 Oxidoreductases

         1.3 Acting on the CH-CH group of donors

             1.3.1 With NAD⁺ or NADP⁺ as acceptor

                1.3.1.12 prephenate dehydrogenase

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Results	in table
8	Activating Compound
11849	AA Sequence
2	Application
3	CAS Registry Number
21	Cloned(Commentary)
37	Cofactor
5	Crystallization (Commentary)
4	General Information
21	General Stability
1	IC50 Value
105	Inhibitors
7	kcat/KM [mM/s]
26	Ki Value [mM]
82	KM Value [mM]
6	Metals/Ions
33	Molecular Weight [Da]
60	Natural Substrates/ Products (Substrates)
277	Organism
8	Pathway
21	PDB ID
11	pH Optimum
3	pH Range
1	pI Value
1	Posttranslational Modification
18	Protein Variants
36	Purification (Commentary)
3	Reaction
4	Reaction Type
80	Reference
29	Specific Activity [micromol/min/mg]
13	Storage Stability
140	Substrates and Products (Substrate)
18	Subunits
79	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
4	Temperature Range [°C]
6	Temperature Stability [°C]
51	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.3.1.12 - prephenate dehydrogenase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-15°C, 0.02% sodium azide, N2-atmosphere, more than 0.1 mg/ml protein

Klebsiella aerogenes

-

390544

-20°C, 0.1 M N-ethylmorpholine, pH 7.0, 21 mM citrate, 10% v/v glycerol, 1 mM EDTA, 1 mM DTT

Escherichia coli

-

390527, 390528

-20°C, 0.1 M Tris-HCl buffer, pH 7.5, 1 mM EDTA, 1 mM DTT, 1 mM tyrosine, 70% w/v ammonium sulfate, several weeks

Escherichia coli

-

390539

-20°C, 50 mM Cl/N-ethylmorpholine pH 7.2, 1 mM EDTA, 1 mM sodium citrate, 0.02% w/v sodium azide, 50% v/v glycerol, 10 mM DTT, N2-atmosphere, at least 3 months

Escherichia coli

-

390522

-20°C, 50 mM phosphate buffer, pH 7.4, at least 0.7 mg/ml protein, 10 months

Cupriavidus necator

-

3542

-80°C, purified recombinant His-tagged wild-type enzyme, complete loss of activity when stored in 0.1 M sodium citrate, pH 7.5, 10% glycerol, 1 mM DTT, or moderate loss in activity when stored in 0.1 M MES, pH 7.5, 0.051 M N-ethylmorpholine, 0.01 M diethanolamine, 1 mM EDTA, 1 mM DTT, 10% glycerol,

Escherichia coli

-

655472

-86°C

Aquifex aeolicus

-

677010

0-4°C, 0.1 M Tris-HCl buffer, pH 7.5, 1 mM EDTA, 1 mM DTT, 1 mM tyrosine, 70% w/v ammonium sulfate, for at least 10 days

Klebsiella aerogenes

-

390542

4°C, -20°C, or -80°C, 50 mM Tris, 25% glycerol, no significant differences in activity, even after one month of storage

Streptococcus mutans

Q8DUW0

726409

4°C, 0.1 M N-ethylmorpholine, pH 7.0, 21 mM citrate, 10% v/v glycerol, 1 mM EDTA, 1 mM DTT, up to 4 weeks

Escherichia coli

-

390527

4°C, 0.1 M phosphate buffer pH 7.6, over-night, complete loss of activity

Escherichia coli

-

390539

4°C, ammonium sulfate precipitate

Escherichia coli

-

390552