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1.3.1.10: enoyl-[acyl-carrier-protein] reductase (NADPH, Si-specific)

This is an abbreviated version!
For detailed information about enoyl-[acyl-carrier-protein] reductase (NADPH, Si-specific), go to the full flat file.

Word Map on EC 1.3.1.10

Reaction

acyl-[acyl-carrier protein]
+
NADP+
=
trans-2,3-dehydroacyl-[acyl-carrier protein]
 +
NADPH
 +
H+

Synonyms

1-cyclohexenylcarbonyl coenzyme A reductase, 2-enoyl thioester reductase, 2-trans-enoyl-ACP reductase, acyl-ACP dehydrogenase, beta-ketoacyl reductase, enoyl acyl-carrier-protein reductase, enoyl reductase, enoyl thioester reductase, enoyl-ACP reductase, enoyl-ACP reductase II, enoyl-ACP reductase III, enoyl-ACP-reductase II, enoyl-acyl carrier protein reductase, enoyl-[acyl carrier protein] reductase, enoyl-[acyl-carrier-protein] reductase, ENR, Etr1p, fabG4, FabI, FabK, FabL, Fabl2, More, Mrf1p, NADPH 2-enoyl Co A reductase, reductase, enoyl-[acyl carrier protein] (reduced nicotinamide adenine dinucleotide phosphate), trans-enoyl-[acyl-carrier-protein] reductase, YgaA

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.10 enoyl-[acyl-carrier-protein] reductase (NADPH, Si-specific)

Purification

Purification on EC 1.3.1.10 - enoyl-[acyl-carrier-protein] reductase (NADPH, Si-specific)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
chromatography of protein overexpressed in Escherichia coli by using Sephadex G-100, DEAE, Mono-Q HR 5/5
-
in BisTris puffer pH 6.5 in order to retain NADPH dependent activity which is lost at pH 7.5
-
Ni-NTA column chromatography and Superdex 200 gel filtration
-
Ni2+ affinity chromatography on his-tagged protein
Ni2+ chelation chromatography
Ni2+-NTA resin chromatography
-
nickel-chelated Hi-Trap column chromatography, Hi-Trap Blue HP column chromatography, Q-Sepharose column chromatography, and Superdex-75 gel filtration
-
no separation from NADH specific enzyme
-
recombinant His6-tagged BcFabL from Escherichia coli strain BL21(DE3) by nickel affinity and adsorption chromatography and gel filtration
recombinant protein
recombinant wild-type enzyme and mutant Y73N from mutant strain BJ1991 mrf1DELTA
recombinant wild-type enzyme and mutant Y79N from Saccharomyces cerevisiae mutant strain BJ1991 mrf1DELTA
-
sonification and chromatography using DEAE-Cellulose, Sephadex G-100, Hydroxylapatite
-
which also catalyzes the reduction of enoyl-CoA substrates
-