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1.21.99.5: tetrachloroethene reductive dehalogenase

This is an abbreviated version!
For detailed information about tetrachloroethene reductive dehalogenase, go to the full flat file.

Word Map on EC 1.21.99.5

Reaction

trichloroethene
+
chloride
+
acceptor
=
tetrachloroethene
+
reduced acceptor

Synonyms

1,2,4,5-TeCB reductive dehalogenase, dceA6, dehalogenase, polychloroethene, EC 1.97.1.8, mbrA, PCE dehalogenase, PCE reductase, PCE reductive dehalogenase, PCE-dechlorinating enzyme, PCE-RD, PCE-RDase, PCE-reductive dehalogenase, PCE/PCB RDase, PCE/TCERD, PceA, pceA gene product, PceA1, PceA2, PceATCE, PceC, pceC gene product, PCER, PCERD, perchloroethylene dehalogenase, polychloroethane dehalogenase, RD11, RD8, RDase, RDH, RdhA, RdhA3, reductive dehalogenase, reductive PCE dehalogenase, Ssed_3769, TcbA, TCE dehalogenase, TCE reductase, tetrachlorobenzene dehalogenase, tetrachloroethene (PCE) dehalogenase, tetrachloroethene (PCE) reductive dehalogenase, tetrachloroethene and trichloroethene dehalogenase, tetrachloroethene dehalogenase, tetrachloroethene reductase, tetrachloroethene reductive dehalogenase, tetrachloroethene reductive dehalogenase catalytic subunit PceA, tetrachloroethylene reductase, ThMA, trichloroethene dehalogenase

ECTree

     1 Oxidoreductases
         1.21 Catalysing the reaction X-H + Y-H = X-Y
             1.21.99 With unknown physiological acceptors
                1.21.99.5 tetrachloroethene reductive dehalogenase

Engineering

Engineering on EC 1.21.99.5 - tetrachloroethene reductive dehalogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R357K
-
the mutant shows about 1% of wild type activity
R357N
-
the mutant shows about 10% of wild type activity
R357Q
-
the mutant shows about 70% of wild type activity
synthesis
the expression of catalytically active PceA enzyme in Shimwellia blattae is significantly enhanced when its dedicated PceT chaperone is coproduced and when 5,6-dimethylbenzimidazole and hydroxocobalamin are added to the cultures
Y298H
-
the mutant shows about 30% of wild type activity
Y298S
-
the mutant shows about 3% of wild type activity
Y298T
-
the mutant shows about 10% of wild type activity
synthesis
-
the expression of catalytically active PceA enzyme in Shimwellia blattae is significantly enhanced when its dedicated PceT chaperone is coproduced and when 5,6-dimethylbenzimidazole and hydroxocobalamin are added to the cultures
-
R357Q
-
the mutant shows about 70% of wild type activity
-
synthesis
-
the expression of catalytically active PceA enzyme in Shimwellia blattae is significantly enhanced when its dedicated PceT chaperone is coproduced and when 5,6-dimethylbenzimidazole and hydroxocobalamin are added to the cultures
-
Y298H
-
the mutant shows about 30% of wild type activity
-
Y298S
-
the mutant shows about 3% of wild type activity
-
Y298T
-
the mutant shows about 10% of wild type activity
-