1.20.99.1: arsenate reductase (donor)

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For detailed information about arsenate reductase (donor), go to the full flat file.

Word Map on EC 1.20.99.1

1.20.99.1

arsenite

asiii

dissimilatory

shewanella

geobacter

groundwater

selenate

paddy

biogeochemical

arsenic-rich

haloalkaliphile

Reaction

Synonyms

Acr2, ACR2.1, ACR2.2, AR, Arr, ArrA, ArsC, arsenate reductase, arsenite oxidase, As(V) reductase, ATQ1, Cdc25B, Cdc25C, EC 1.97.1.6, gene arsC proteins, HAC1, More, proteins (specific proteins and subclasses), genearsC, reductase, arsenate, respiratory arsenate reductase

ECTree

1 Oxidoreductases

1.20 Acting on phosphorus or arsenic in donors

1.20.99 With unknown physiological acceptors

1.20.99.1 arsenate reductase (donor)

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Results	in table
17	Activating Compound
9	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
6	Cofactor
9	Crystallization (Commentary)
3	Expression
5	General Information
4	kcat/KM [mM/s]
14	KM Value [mM]
3	Localization
17	Metals/Ions
13	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
50	Organism
2	Pathway
4	pH Optimum
1	pH Range
6	Protein Variants
6	Purification (Commentary)
7	Reaction
1	Reaction Type
47	Reference
11	Source Tissue
5	Specific Activity [micromol/min/mg]
67	Substrates and Products (Substrate)
9	Subunits
57	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
12	Turnover Number [1/s]

Purification

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Purification on EC 1.20.99.1 - arsenate reductase (donor)

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Chrysiogenes arsenatis

287859

57.5fold to homogeneity from the periplasm by preparation of spheroplasts, ammonium sulfate fractionation, ultrafiltration, cation exchange chromatography, and gel filtration, purification via anion exchange or hydrophobic interaction chromatography is not successful

Hydrogenophaga sp. NT14

658195

by nickel-nitrilotriacetic acid chromatography

partial

recombinant soluble wild-type and SeMet-labeled enzyme from Escherichia coli

Bacillus subtilis

657473

using Ni-NTA chromatography

Homo sapiens

714217