1.20.99.1: arsenate reductase (donor)

This is an abbreviated version!
For detailed information about arsenate reductase (donor), go to the full flat file.

Reaction

Synonyms

Acr2, ACR2.1, ACR2.2, AR, Arr, ArrA, ArsC, arsenate reductase, arsenite oxidase, As(V) reductase, ATQ1, Cdc25B, Cdc25C, EC 1.97.1.6, gene arsC proteins, HAC1, More, proteins (specific proteins and subclasses), genearsC, reductase, arsenate, respiratory arsenate reductase

ECTree

     1 Oxidoreductases

         1.20 Acting on phosphorus or arsenic in donors

             1.20.99 With unknown physiological acceptors

                1.20.99.1 arsenate reductase (donor)

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Results	in table
17	Activating Compound
9	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
6	Cofactor
9	Crystallization (Commentary)
3	Expression
5	General Information
4	kcat/KM [mM/s]
14	KM Value [mM]
3	Localization
17	Metals/Ions
13	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
50	Organism
2	Pathway
4	pH Optimum
1	pH Range
6	Protein Variants
6	Purification (Commentary)
7	Reaction
1	Reaction Type
47	Reference
11	Source Tissue
5	Specific Activity [micromol/min/mg]
67	Substrates and Products (Substrate)
9	Subunits
57	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
12	Turnover Number [1/s]

Engineering

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Engineering on EC 1.20.99.1 - arsenate reductase (donor)

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

R60A

Escherichia coli

-

site-directed muatgenesis, crystal structure determination and analysis with bound arsenite, formation of a covalent and more stableCys12-thiol-dihydroxyarsenite intermediate compared to the wild-type enzyme, resulting in slow release of product and loss of activity

660461

R60K

Escherichia coli

-

site-directed muatgenesis, crystal structure determination and analysis with bound arsenite, formation of a covalent and more stableCys12-thiol-dihydroxyarsenite intermediate compared to the wild-type enzyme, resulting in slow release of product and loss of activity

660461

C120A

Homo sapiens

-

inactive mutant

714217

R126A

Homo sapiens

-

inactive mutant

714217

C71S

Oryza sativa

Q10SX6, Q336V5

mutagenesis of cysteine residues in the putative active site HC(X) 5 R motif leads to nearly complete loss of both phosphatase and arsenate reductase activities

694407

additional information

Homo sapiens

-

catalytic domain of Cdc25B is expressed in bacteria. Cdc25B shows arsenate reductase activity. Enzyme reduces inorganic arsenate but not methylated pentavalent arsenicals. Alteration of either the cysteine and arginine residues of the Cys-X5-Arg motif leads to the loss of both reductase and phosphatase activities

714217