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1.20.4.1: arsenate reductase (glutathione/glutaredoxin)

This is an abbreviated version!
For detailed information about arsenate reductase (glutathione/glutaredoxin), go to the full flat file.

Word Map on EC 1.20.4.1

Reaction

arsenate
+
glutathione
+
glutaredoxin
=
arsenite
+
a glutaredoxin-glutathione disulfide
+
H2O

Synonyms

Acr2, Acr2p, ACR3-ArsC, All0195, AR, ARSA, ArsC, arsenate reductase, arsenate reductase (glutaredoxin), AsV reductase, EC 1.97.1.5, gene arsC proteins, glutaredoxin, glutathione:arsenate oxidoreductase, Grx, MXAN_0575, PcAcr2, proteins (specific proteins and subclasses), gene arsC, PvGRX5, reductase, arsenate, slr0946, SynArsC

ECTree

     1 Oxidoreductases
         1.20 Acting on phosphorus or arsenic in donors
             1.20.4 With disulfide as acceptor
                1.20.4.1 arsenate reductase (glutathione/glutaredoxin)

Engineering

Engineering on EC 1.20.4.1 - arsenate reductase (glutathione/glutaredoxin)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A11W
-
same Km for arsenate as the wild type with maximal velocity approximately half that of the wild type enzyme
A11W/C12S
-
catalytic inactive mutation
C106G
-
the maximal velocity is approximately half that of the wild type enzyme
C12S
-
catalytically inactive mutant
Y7W
-
same Km and maximal velocity as the wild type
C67A
loss of both GRX activity and arsenic resistance
N13A
decrease in activity due to lower local softness, analysis of S-As bond length, Wiberg bond orders, lewaving group energy and nucleofugality
R16A
decrease in activity due to lower local softness, analysis of S-As bond length, Wiberg bond orders, lewaving group energy and nucleofugality
S17A
decrease in activity due to lower local softness, analysis of S-As bond length, Wiberg bond orders, lewaving group energy and nucleofugality
C13A
114% of wild-type vmax
C35A
l00% of wild-type vmax
C80A
less than 2% of wild-type vmax
C80A/C82A
less than 2% of wild-type vmax
C82A
less than 2% of wild-type vmax
C8A
less than 2% of wild-type vmax
additional information