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0.0026
NAD+
pH 7.2, 30°C
0.0045
NAD+
-
pH 7.3, 25°C
0.016
NAD+
-
25°C, pH 7.25, E175A mutant enzyme
0.017
NAD+
mutant enzyme M53A, at pH 7.3 and 25°C
0.0194
NAD+
-
pH 7.3, 25°C
0.02
NAD+
-
25°C, pH 7.25, E175A/A176R mutant enzyme
0.022
NAD+
mutant 17X-PTDH, pH 7.3, 25°C
0.022
NAD+
mutant enzyme E175A, at pH 7.3 and 25°C
0.032
NAD+
-
mutant M26I/E332N/C336D, pH 7.3, 25°C
0.034
NAD+
-
mutant D13E/M26I/E332N/C336D, pH 7.3, 25°C
0.036
NAD+
mutant Y139F, pH 7.3, 25°C
0.037
NAD+
-
mutant K330*, pH 7.3, 25°C
0.039
NAD+
-
mutant D13E/M26I/E175A/T181S/E332N/C336D, pH 7.3, 25°C
0.04
NAD+
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, pH 7.3, 25°C
0.04
NAD+
12 x PTDH mutant
0.0405
NAD+
-
pH 7.3, 25°C
0.041
NAD+
-
mutant D13E/M26I/E175A/E332N/C336D, pH 7.3, 25°C
0.045
NAD+
-
mutant D13E/M26I/E175A/T181S/A308T/E332N/C336D, pH 7.3, 25°C
0.046
NAD+
-
mutant Q132R/Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C
0.05
NAD+
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V, pH 7.3, 25°C
0.052
NAD+
-
mutant Q137R/I150F/Q215L/R275Q/A319E, pH 7.3, 25°C
0.0522
NAD+
wild-type, pH 7.3, 25°C
0.053
NAD+
-
25°C, pH 7.25, wild type enzyme
0.053
NAD+
-
wild-type, pH 7.3, 25°C
0.056
NAD+
-
pH 7.2, 30°C
0.056
NAD+
-
mutant Q137R/I150F/Q215L/R275Q/L276Q, pH 7.3, 25°C
0.057
NAD+
-
pH 7.5, 25°C, wild type enzyme
0.057
NAD+
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R, pH 7.3, 25°C
0.06
NAD+
-
25°C, pH 7.25, A176R mutant enzyme
0.06
NAD+
-
mutant Q132R, pH 7.3, 25°C
0.063
NAD+
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I, pH 7.3, 25°C
0.064
NAD+
-
mutant Q215L, pH 7.3, 25°C
0.064
NAD+
mutant enzyme M53N, at pH 7.3 and 25°C
0.065
NAD+
-
mutant Q137R/I150F/Q215L/R275Q/D162N/V315A, pH 7.3, 25°C
0.066
NAD+
-
mutant Q137R, pH 7.3, 25°C
0.066
NAD+
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A, pH 7.3, 25°C
0.066
NAD+
-
wild-type, pH 7.3, 25°C
0.07
NAD+
-
mutant R275Q, pH 7.3, 25°C
0.071
NAD+
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L, pH 7.3, 25°C
0.074
NAD+
-
mutant Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C
0.074
NAD+
mutant R301K, pH 7.3, 25°C
0.075
NAD+
-
mutant I150F, pH 7.3, 25°C
0.075
NAD+
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K, pH 7.3, 25°C
0.102
NAD+
mutant W134F, pH 7.3, 25°C
0.11
NAD+
-
pH 7.5, 25°C, K76R mutant enzyme
0.128
NAD+
-
pH 7.2, 40°C
0.187
NAD+
mutant W134A, pH 7.3, 25°C
0.2
NAD+
-
pH 7.5, 25°C, K76C mutant enzyme
0.216
NAD+
mutant R301A, pH 7.3, 25°C
0.25
NAD+
mutant S295A, pH 7.3, 25°C
0.26
NAD+
-
pH 7.5, 25°C, K76A mutant enzyme
0.9
NAD+
-
pH 7.5, 25°C, K76M mutant enzyme
1
NAD+
-
pH 7.5, 25°C, E266Q mutant enzyme
1
NAD+
-
pH 7.5, 25°C, R237K mutant enzyme
0.004
NADP+
-
25°C, pH 7.25, E175A/A176R mutant enzyme
0.056
NADP+
-
mutant D13E/M26I/E175A/T181S/A308T/E332N/C336D, pH 7.3, 25°C
0.057
NADP+
-
mutant D13E/M26I/E175A/T181S/E332N/C336D, pH 7.3, 25°C
0.065
NADP+
-
mutant D13E/M26I/E175A/E332N/C336D, pH 7.3, 25°C
0.077
NADP+
-
25°C, pH 7.25, A176R mutant enzyme
0.14
NADP+
-
mutant E175A, pH 7.3, 25°C
0.144
NADP+
-
25°C, pH 7.25, E175A mutant enzyme
1.07
NADP+
-
mutant D13E/M26I/E332N/C336D, pH 7.3, 25°C
2.5
NADP+
-
wild-type, pH 7.3, 25°C
2.51
NADP+
-
25°C, pH 7.25, wild type enzyme
0.001
phosphite
-
mutant R237K
0.014
phosphite
-
cosubstrate NAD+, mutant K330*, pH 7.3, 25°C
0.027
phosphite
-
cosubstrate NAD+, mutant M26I/E332N/C336D, pH 7.3, 25°C
0.03
phosphite
-
cosubstrate NAD+, mutant D13E/M26I/E332N/C336D, pH 7.3, 25°C
0.035
phosphite
-
mutant D79N
0.045
phosphite
-
mutant Q132R, pH 7.3, 25°C
0.046
phosphite
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V, pH 7.3, 25°C
0.046
phosphite
12 x PTDH mutant
0.047
phosphite
-
cosubstrate NAD+, wild-type, pH 7.3, 25°C
0.048
phosphite
-
cosubstrate NAD+, mutant D13E/M26I/E175A/T181S/A308T/E332N/C336D, pH 7.3, 25°C
0.048
phosphite
-
mutant Q137R, pH 7.3, 25°C
0.055
phosphite
-
wild-type
0.057
phosphite
-
wild-type, pH 7.3, 25°C
0.057
phosphite
parent enzyme
0.058
phosphite
-
mutant Q215L, pH 7.3, 25°C
0.062
phosphite
-
cosubstrate NADP+, mutant D13E/M26I/E175A/T181S/E332N/C336D, pH 7.3, 25°C
0.07
phosphite
-
cosubstrate NADP+, mutant D13E/M26I/E175A/T181S/A308T/E332N/C336D, pH 7.3, 25°C
0.075
phosphite
-
mutant Q132R/Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C
0.078
phosphite
-
mutant R275Q, pH 7.3, 25°C
0.079
phosphite
Opt12 variant
0.082
phosphite
-
cosubstrate NAD+, mutant D13E/M26I/E175A/E332N/C336D, pH 7.3, 25°C
0.084
phosphite
-
cosubstrate NAD+, mutant D13E/M26I/E175A/T181S/E332N/C336D, pH 7.3, 25°C
0.087
phosphite
-
cosubstrate NADP+, mutant D13E/M26I/E175A/E332N/C336D, pH 7.3, 25°C
0.09
phosphite
Opt13 variant
0.092
phosphite
-
mutant Q137R/I150F/Q215L/R275Q/A319E, pH 7.3, 25°C
0.096
phosphite
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A, pH 7.3, 25°C
0.099
phosphite
-
mutant I150F, pH 7.3, 25°C
0.105
phosphite
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I, pH 7.3, 25°C
0.12
phosphite
-
mutant Q137R/I150F/Q215L/R275Q/L276Q, pH 7.3, 25°C
0.123
phosphite
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K, pH 7.3, 25°C
0.135
phosphite
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V, pH 7.3, 25°C
0.138
phosphite
-
cosubstrate NADP+, mutant E175A, pH 7.3, 25°C
0.142
phosphite
Opt14 variant
0.144
phosphite
-
mutant Q137R/I150F/Q215L/R275Q, pH 7.3, 25°C
0.155
phosphite
-
mutant Q137R/I150F/Q215L/R275Q/D162N/V315A, pH 7.3, 25°C
0.156
phosphite
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L, pH 7.3, 25°C
0.169
phosphite
-
mutant Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R, pH 7.3, 25°C
0.475
phosphite
pH 7.0, 25°C
1.2
phosphite
-
mutant D79A
1.9
phosphite
-
cosubstrate NADP+, mutant D13E/M26I/E332N/C336D, pH 7.3, 25°C
1.9
phosphite
-
cosubstrate NADP+, wild-type, pH 7.3, 25°C
0.0073
phosphonate
-
pH 7.2, 30°C
0.0087
phosphonate
pH 7.2, 30°C
0.017
phosphonate
mutant enzyme M53N, at pH 7.3 and 25°C
0.024
phosphonate
pH 7.2, 40°C
0.0254
phosphonate
-
pH 7.3, 25°C
0.028
phosphonate
mutant 17X-PTDH, pH 7.3, 25°C
0.028
phosphonate
wild type enzyme, at pH 7.3 and 25°C
0.028
phosphonate
mutant enzyme E175A, at pH 7.3 and 25°C
0.039
phosphonate
mutant Y139F, pH 7.3, 25°C
0.053
phosphonate
-
pH 7.5, 25°C, wild type enzyme
0.0549
phosphonate
-
pH 7.3, 25°C
0.0811
phosphonate
wild-type, pH 7.3, 25°C
0.1
phosphonate
mutant enzyme M53A, at pH 7.3 and 25°C
0.103
phosphonate
mutant S295A, pH 7.3, 25°C
0.201
phosphonate
-
at pH 7.5 and 30°C
0.237
phosphonate
-
pH 7.3, 25°C
0.238
phosphonate
mutant W134F, pH 7.3, 25°C
0.287
phosphonate
-
pH 7.2, 40°C
0.35
phosphonate
-
pH 7.5, 25°C, K76R mutant enzyme
0.6
phosphonate
-
pH 7.5, 25°C, K76C mutant enzyme
1.1
phosphonate
-
pH 7.5, 25°C, K76A mutant enzyme
1.1
phosphonate
mutant enzyme R301K, at pH 7.3 and 25°C
1.11
phosphonate
mutant R301K, pH 7.3, 25°C
1.91
phosphonate
mutant W134A, pH 7.3, 25°C
2
phosphonate
-
pH 7.5, 25°C, K76M mutant enzyme
5
phosphonate
-
pH 7.5, 25°C, E266Q mutant enzyme
6
phosphonate
-
pH 7.5, 25°C, R237K mutant enzyme
19.4
phosphonate
mutant R301A, pH 7.3, 25°C
20
phosphonate
mutant enzyme R301A, at pH 7.3 and 25°C
1.20.1.1 phosphonate dehydrogenase
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results (
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