1.20.1.1: phosphonate dehydrogenase

This is an abbreviated version!
For detailed information about phosphonate dehydrogenase, go to the full flat file.

Reaction

Synonyms

NAD-dependent phosphite dehydrogenase, NAD:phosphite oxidoreductase, phosphite dehydrogenase, PTDH, PtxD

ECTree

     1 Oxidoreductases

         1.20 Acting on phosphorus or arsenic in donors

             1.20.1 With NAD⁺ or NADP⁺ as acceptor

                1.20.1.1 phosphonate dehydrogenase

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Results	in table
103	AA Sequence
10	Application
1	CAS Registry Number
16	Cloned(Commentary)
13	Cofactor
3	Crystallization (Commentary)
33	Inhibitors
11	kcat/KM [mM/s]
5	Ki Value [mM]
146	KM Value [mM]
2	Localization
3	Metals/Ions
6	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
62	Organism
4	PDB ID
5	pH Optimum
5	pH Range
1	pH Stability
4	pI Value
150	Protein Variants
9	Purification (Commentary)
3	Reaction
1	Reaction Type
36	Reference
6	Specific Activity [micromol/min/mg]
1	Storage Stability
51	Substrates and Products (Substrate)
6	Subunits
36	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
3	Temperature Range [°C]
8	Temperature Stability [°C]
76	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.20.1.1 - phosphonate dehydrogenase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

analysis of the electrostatic potentials of the interfaces between the subunits. Regions A, B, and C are almost electroneutral

Pseudomonas sp.

F2YGD2

726694

mutant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336D, to 2.3 A resolution. The TS-PTDH monomer may be divided into a large and a small domain, separated by a flexible hinge region. The NAD+ cofactor is housed at the junction between the two domains, where residues from the large subunit engage in interactions with the ligand. In the cocrystal structure with NAD+ and inhibitor sulfite, the sulfite anion is situated proximal to the nicotinamide of the cofactor, where it is engaged through interactions with the side chains of Arg237, His292, and the backbone amides of Lys76 and Gly77

Pseudomonas stutzeri

O69054

726982

mutant enzymes R301K and R301A, hanging drop vapor diffusion method, using 0.1 M NaCl, 0.1 M sodium cacodylate pH 5.8, 16% PEG 6000 (w/v) (for mutant R301K) or 0.1 M ammonium acetate, 0.1 M Bis Tris pH 5.5, 17% PEG 10000 (w/v) (for mutant R301A)

Pseudomonas stutzeri

O69054

746256