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1.2.4.4: 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)

This is an abbreviated version!
For detailed information about 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring), go to the full flat file.

Word Map on EC 1.2.4.4

Reaction

3-methyl-2-oxobutanoate
+
[dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine
=
[dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine
+
CO2
+ 2 H+

Synonyms

2-oxoisocaproate dehydrogenase, 2-oxoisovalerate dehydrogenase, 2-oxoisovalerate dehydrogenase (lipoate), 2-oxoisovalerate dehydrogenase subunit alpha, alpha-keto-beta-methylvalerate dehydrogenase, alpha-ketoacid dehydrogenase, alpha-ketoisocaproate dehydrogenase, alpha-ketoisocaproic dehydrogenase, alpha-ketoisocaproic-alpha-keto-beta-methylvaleric dehydrogenase, alpha-ketoisovalerate dehydrogenase, alpha-oxoisocaproate dehydrogenase, BCDH, BCKA dehydrogenase, BCKADC, BCKD, BCKDC, BCKDH, BCKDH E1-alpha, BCKDH E1-beta, BCKDHA, BCKDHB, BCKDHc, BCOA, BCOAD, BDKG, Bkd, bkdF, branched chain alpha-ketoacid dehydrogenase complex, branched chain keto acid dehydrogenase, branched-chain (-2-oxoacid BCD) dehydrogenase, branched-chain 2-keto acid dehydrogenase, branched-chain 2-oxo acid dehydrogenase, branched-chain alpha-keto acid decarboxylase/dehydrogenase, branched-chain alpha-keto acid dehydrogenase, branched-chain alpha-keto acid dehydrogenase complex, branched-chain alpha-keto acid dehydrogenase E1beta subunit, branched-chain alpha-ketoacid dehydrogenase, branched-chain alpha-ketoacid dehydrogenase complex, branched-chain alpha-ketoacid dehydrogenase enzyme complex, branched-chain alpha-ketoacid dehydrogenase multienzyme complex, branched-chain alpha-oxo acid dehydrogenase, branched-chain keto acid dehydrogenase, branched-chain keto acid dehydrogenase complex, branched-chain ketoacid dehydrogenase, branched-chain ketoacid dehydrogenase E1, dehydrogenase, 2-oxoisocaproate, dehydrogenase, 2-oxoisovalerate (lipoate), dehydrogenase, branched chain alpha-keto acid, DHalpha, dihydrolipoyl acyl-transferase, dihydrolipoyl transacylase, E1b, E1b component, E1b component of the 2-oxo acid dehydrogenase complex, E2, EC 1.2.4.3, More

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.4 With a disulfide as acceptor
                1.2.4.4 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)

Cloned

Cloned on EC 1.2.4.4 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
beta-subunit
expressed in Escherichia coli BL-21 cells
-
expressed in Escherichia coli BL21(DE3) cells and BL21(DE3)pLysS cells
-
expressed in Escherichia coli strain DH5alpha
-
expression in Escherichia coli
expression in Pseudomonas putida and Escherichia coli
-
expression of alpha- and beta-subunits of component E1 in Escherichia coli
-
expression of C-terminally His-tagged lipoic acid-bearing domain LBD, amino acid residues 1-84 of E2b
-
expression of C-terminally His-tagged lipoic acid-bearing domain of enzyme complex component E2 with an extra methionine at the N-terminus and a glutamic acid and leucine at the C-terminus in Escherichia coli strain BL21(DE3)
-
expression of E1 component in Escherichia coli as a homotetramer, in Escherichia coli only one translational start site for E1beta is recognized and only the 39000 Da product is produced, in addition the N-terminal amino acid of the 39000 Da E1beta subunit is Met instead of Leu
-
expression of N-and C-terminally His6-tagged component E1b and mutants, and of C-terminally His-tagged lipoic acid-bearing domain, amino acid residues 1-84 of component E2b, in vitro lipoylation of the latter
-
expression of the lipoic acid-bearing domain of complex component E2 in Escherichia coli strain BL21(DE3) as His-tagged protein
-
overexpression of bacterial branched-chain alpha-keto acid dehydrogenase multienzyme complex BCKDC, encoded by 4 genes, in Arabidopsis thaliana targeted to chloroplasts, about 7fold increased enzyme activity in the transgenic plants
-
recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with superoxide dismutase of Thermus thermophilus in Escherichia coli, interaction analysis of 2-oxoisovalerate dehydrogenase alpha subunit with superoxid dismutase of Thermus thermophilus by yeast two hybrid system, recombinant expression in Saccharomyces cerevisiae
region containing the genes for the entire enzyme complex
-