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1.2.3.3: pyruvate oxidase

This is an abbreviated version!
For detailed information about pyruvate oxidase, go to the full flat file.

Word Map on EC 1.2.3.3

Reaction

pyruvate
+
phosphate
+
O2
=
acetyl phosphate
+
CO2
+
H2O2

Synonyms

acetyl phosphate-producing pyruvate oxidase, phosphate-dependent pyruvate oxidase, POD, POX, POX-2, poxB, PoxF, PyOD, PyrOx, pyruvate oxidase, pyruvate:oxygen-2-oxidoreductase (phosphorylating), pyruvic oxidase, SpxB, SpxB protein

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.3 With oxygen as acceptor
                1.2.3.3 pyruvate oxidase

Purification

Purification on EC 1.2.3.3 - pyruvate oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by anion-exchange chromatography, more than 95% pure
-
by gel filtration
ion exchange column chromatography, immobilization on aminoalkylated controlled pore glass by crosslinking with glutaraldehyde
-
to homogeneity, ultracentrifugation and chromatography techniques
-