1.2.1.46: formaldehyde dehydrogenase

This is an abbreviated version!
For detailed information about formaldehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.46

1.2.1.46

methanol

gsh-dependent

s-hydroxymethylglutathione

boidinii

hyphomicrobium

s-formylglutathione

methanol-utilizing

1.2.1.1

methanol-grown

industry

formaldehyde-induced

analysis

degradation

biotechnology

Reaction

Synonyms

ADH3, dehydrogenase, formaldehyde, DL-FDH, dlFalDH, dye-linked formaldehyde dehydrogenase, FADH, FALDH, FdDH, FDH, FDH1, fdhA, FldDH, Fldh1, formaldehyde dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase, glutathione-independent FDH, glutathione-independent formaldehyde dehydrogenase, GSH-FDH/ADH, NAD+- and glutathione-dependent formaldehyde dehydrogenase, NAD+- and glutathione-independent formaldehyde dehydrogenase, NAD+- and glutathione_dependent formaldehyde dehydrogenase, NAD+-dependent formaldehyde dehydrogenase, NAD-dependent formaldehyde dehydrogenase, NAD-linked formaldehyde dehydrogenase

ECTree

1 Oxidoreductases

1.2 Acting on the aldehyde or oxo group of donors

1.2.1 With NAD⁺ or NADP⁺ as acceptor

1.2.1.46 formaldehyde dehydrogenase

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Results	in table
6	Activating Compound
1863	AA Sequence
14	Application
1	CAS Registry Number
9	Cloned(Commentary)
40	Cofactor
4	Crystallization (Commentary)
10	Disease/ Diagnostics
2	Expression
8	General Information
6	General Stability
39	Inhibitors
6	kcat/KM [mM/s]
13	Ki Value [mM]
38	KM Value [mM]
1	Localization
18	Metals/Ions
14	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
115	Organism
6	Pathway
2	PDB ID
14	pH Optimum
3	pH Range
4	pH Stability
4	Protein Variants
16	Purification (Commentary)
4	Reaction
6	Reaction Type
52	Reference
9	Source Tissue
15	Specific Activity [micromol/min/mg]
6	Storage Stability
95	Substrates and Products (Substrate)
23	Subunits
63	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Range [°C]
4	Temperature Stability [°C]
5	Turnover Number [1/s]

Purification

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Purification on EC 1.2.1.46 - formaldehyde dehydrogenase

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2 entries

by ion-exchange chromatography

Ogataea angusta

685697, 690067

by two-step anion-exchange chromatography and precipitation by 80% (NH4)2SO4, precipitate, 3.8fold purified

C-83

DE52, Q-sepharose and Sephacryl S-100HR chromatographic steps

Homo sapiens

667724

FdDH ss isolated from the cell-free extract by a two-step ion exchange chromatography

Ogataea angusta

701261

nickel chelate affinity column chromatography and Superdex 200 gel filtration

partial

partially purified

Hyphomicrobium zavarzinii

690106

recombinant His-tagged enzyme 94.1fold from Escherichia coli strain BL21(DE3)/pG-TF2 by nickel affinity chromatography dialysis, anion exchange chromatography, and gelfiltration

Pseudomonas aeruginosa

743745

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)/pG-TF2 by nickel affinity chromatography dialysis, anion exchange chromatography, and dialysis against crystallization buffer

Pseudomonas aeruginosa

Q9HTE3

741521

recombinant protein, expressed in Escherichia coli

Pseudomonas putida

288253