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1.2.1.44: cinnamoyl-CoA reductase

This is an abbreviated version!
For detailed information about cinnamoyl-CoA reductase, go to the full flat file.

Word Map on EC 1.2.1.44

Reaction

cinnamaldehyde
+
CoA
 +
NADP+
=
cinnamoyl-CoA
 +
NADPH
 +
H+

Synonyms

AtCCR1, BpCCR1, CCR, CCR1, CCR12, CCR14 isoform, CCR17, CCR19, CCR2, CCR2-1, CCR20, CCR21, CCR3, CCR7, CCRH1, cinnamoyl CoA reductase, cinnamoyl CoA reductase 1, cinnamoyl coenzyme A reductase, cinnamoyl-Co-enzyme A reductase, cinnamoyl-CoA reductase, cinnamoyl-CoA reductase 1, cinnamoyl-CoA reductase1, cinnamoyl-CoA reductase2, cinnamoyl-CoA:NADPH reductase, cinnamoyl-coenzyme A reductase, cinnamoyl-coenzyme A reductase 1, FaCCR, feruloyl coenzyme A reductase, feruloyl-CoA reductase, ferulyl-CoA reductase, HcCCR1, HcCCR2, Ll-CCRH1, OsCCR1, p-hydroxycinnamoyl coenzyme A reductase, Ph-CCR1, PtCCR, PtoCCR1, PtoCCR7, PvCCR1, PvCCR2, reductase, cinnamoyl coenzyme A, SbCCR1, SbCCR2-1, SbCCR2-2, Ta-CCR2, VcCCR

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.44 cinnamoyl-CoA reductase

Cloned

Cloned on EC 1.2.1.44 - cinnamoyl-CoA reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CCR, recombinant expression of His-tagged enzyme CCR in Escherichia coli, coexpression with 4-coumarate-CoA ligase, EC 6.2.1.12. The best expression conditions for recombinant PtCCR are 6 h of 0.4 mM IPTG induction at 37°C
cloned into the pET-29b vector and expressed in Escherichia coli strains BL21(DE3), BL21(DE3)pLysS, RIL, and RP
expressed in Escherichia coli BL21 (DE34) cells
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli M15 cells
expressed in Nicotiana benthamiana
-
expressed in Nicotiana tabacum variety Xanthi via Agrobacterium tumefaciens strain LBA4404
-
five-year-old transgenic plants of Norway spruce Picea abies expressing the Norway spruce gene encoding CCR in antisense orientation under the control of the maize ubiquitin promoter
for generation of RNAi constructs and for tomato transformation through Agrobacterium tumefaciens, into the pGEX4T-1 vector for expression in Escherichia coli BL21 cells
for the construction of a yeast two-hybrid assay prey vectors containing different fragments of OsCCR1 are made by using the pVP16 vector, for transient expression in rice protoplasts a OsCCR1-GFP construct is generated, full length OsCCR1 is cloned into a pET30 vector for expression in Escherichia coli cells
-
gene CCR, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression analysis by differential display reverse transcription-polymerase chain reaction, DDRT-PCR, recombinant expression in Escherichia coli
gene CCR, recombinant overexpression of chimeric 4CL1-CCR in Escherichia coli strain M15 supplemented with phenylpropanoic acids. Three 4-hydroxycinnamaldehydes, p-coumaraldehyde, caffealdehyde and coniferaldehyde, are thereby biosynthesized and secreted into the culture medium, identification of phenylpropanoic acids and 4-hydroxycinnamaldehydes by HPLC–PDA–ESI–MSn
gene CCR, sequence comparisons and phylogenetic analysis
gene CCR1, DNA and amino acid sequence determination and analysis of diverse isozymes from Populus tomentosa, phylogenetic analysis, recombinant expression of His-tagged isozyme CCR1 in Escherichia coli strain M15, subcloning in Escherichia coli strain DH5alpha
gene CCR1, DNA and amino acid sequence determination and analysis, phylogenetic analysis
Betula platyphylla x Betula pendula
gene CCR1, recombinant expression of His6-tagged enzyme in Escherichia coli
gene CCR1, recombinant His-tagged enzyme expression in Escherichia coli strain Rosetta (DE3)
gene CCR1, the gene locus maps to chromosome 1, DNA and amino acid sequence determination and analysis
-
gene CCR7, DNA and amino acid sequence determination and analysis of diverse isozymes from Populus tomentosa, phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant isozymes CCR7 in Escherichia coli strain M15, subcloning in Escherichia coli strain DH5alpha
gene FaCCR, DNA and amino acid sequence determination and analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
gene HcCCR1, sequence comparisons and phylogenetic analysis, quantitative gene expression analysis
gene SbCCR1, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strain DH5alpha, phylogenetic analysis, recombinant expression of GFP-tagged enzyme in Nicotiana benthaminana leaves via Agrobacterum tumefaciens transfection, quantitative real-time PCR enzyme expression analysis
gene SbCCR2-1, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strain DH5alpha, phylogenetic analysis, recombinant expression of GFP-tagged enzyme in Nicotiana benthaminana leaves via Agrobacterum tumefaciens transfection, quantitative real-time PCR enzyme expression analysis
gene SbCCR2-2, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strain DH5alpha, phylogenetic analysis, recombinant expression of GFP-tagged enzyme in Nicotiana benthaminana leaves via Agrobacterum tumefaciens transfection, quantitative real-time PCR enzyme expression analysis
heterologous expression as His-tagged fusion protein in Escherichia coli
into the pET41a+ and pET23b+ vector for expression in Escherichia coli BL21DE3 cells
into the pGEX-4T vector for expression in Escherichia coli BL21DE3 cells
-
introduction of four different constructs into poplar (Populus tremula x Populus alba)
isoforms CCR17, CCR19, CCR20, and CCR21 are expressed in Escherichia coli BL21(DE3) cells
-
production of active enzyme in Escherichia coli
-
specific PCR product is cloned into the pGEM-T Easy vector, as an expression vector for Escherichia coli, pET28a is used
two genes AtCCR1 and AtCCR2, localised on chromosome 1, AtCCR1 is associated with lignin biosynthesis during development, AtCCR2 is associated with the hypersensitive response to pathogen attack
-
two genes ZmCCR1 and ZmCCR2, ZmCCR1 is involved in constitutive lignification, function of ZmCCR2 unknown
-
two recombinant proteins expressed in Escherichia coli
-