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1.2.1.28: benzaldehyde dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about benzaldehyde dehydrogenase (NAD+), go to the full flat file.

Reaction

benzaldehyde
+
NAD+
+
H2O
=
benzoate
+
NADH
+
H+

Synonyms

BALDH, benzaldehyde dehydrogenase, benzaldehyde dehydrogenase (NAD), BZDH, mdlD, More, NAD(P)-dependent benzaldehyde dehydrogenase, PpBADH, XylC

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.28 benzaldehyde dehydrogenase (NAD+)

Cloned

Cloned on EC 1.2.1.28 - benzaldehyde dehydrogenase (NAD+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli DH10B
-
gene mdlD, recombinant epression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene xylC, recombinant expression in Escherichia coli strain BL21(DE3) in inclusion bodies. Using higher concentration of lysozyme in lysis buffer, high speed, and long-term centrifugation reduces the protein aggregation
-
into the vector pET-28a+ for expression in Escherichia coli BL21 Rosetta cells, into the pEF1-LIS binary vector for transformation of petunia flowers with Agrobacterium tumefaciens, for subcellular localization experiments in Arabidopsis protoplasts GFP fusion proteins are constructed using the vector p326-SGFP
-