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1.2.1.12: glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)

This is an abbreviated version!
For detailed information about glyceraldehyde-3-phosphate dehydrogenase (phosphorylating), go to the full flat file.

Word Map on EC 1.2.1.12

Reaction

D-glyceraldehyde 3-phosphate
+
phosphate
 +
NAD+
=
3-phospho-D-glyceroyl phosphate
 +
NADH
 +
H+

Synonyms

3-phosphoglyceraldehyde dehydrogenase, A4-GAPDH, A4-glyceraldehyde-3-phosphate dehydrogenase, AB-GAPDH, AnBn-GAPDH, AsGAPDH, At3g04120, BARS-38, CbbG, CgGAP, Clo1313_2095, complement-C3-binding protein, CP 17/CP 18, Ctherm_Gapdh, cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase, cytosolic phosphorylating glyceraldehyde-3-phosphate dehydrogenase, D-glyceraldehyde-3-phosphate dehydrogenase, D-glyceraldehyde-3-phosphate: NAD+ oxidoreductase (phosphorylating), dehydrogenase, glyceraldehyde phosphate, dihydrogenase, glyceraldehyde phosphate, EcGAPDH, EcGAPDH1, FgGAPDH, FhGAPDH, G3PD, G3PDH, Ga3P dehydrogenase, Ga3PDHase, GADPH, GAP, GAP1, gap2, GapA, GapB, GAPC, GapC-1, GapC1, GapC2, GAPCp, GAPCp1, GAPCp2, GAPD, GAPDH, GAPDH type 1, GAPDH1, GAPDH2, GAPDH3, GAPDHS, GAPDS, GAPN, GBS GAPDH, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase-S, glyceraldehyde phosphate dehydrogenase (NAD), glyceraldehyde-3 phosphate dehydrogenase, glyceraldehyde-3-P-dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase (NAD), glyceraldehyde-3-phosphate dehydrogenase 1, glyceraldehyde-3-phosphate dehydrogenase, type I, glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein, glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS, glyceraldehyde-3-phosphate dehydrogenases, GPD, GPD2, Gra3PDH, GraP-DH, H.c-C3BP, hGAPDH, HsGAPDH, kmGAPDH1p, Larval antigen OVB95, Major larval surface antigen, Mtb-GAPDH, NAD+-dependent GAPDH, NAD+-dependent glyceraldehyde 3-phosphate dehydrogenase, NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase, NAD+-G-3-P dehydrogenase, NAD+-GAPDH, NAD-dependent Ga3PDHase, NAD-dependent glyceraldehyde 3-phosphate dehydrogenase, NAD-dependent glyceraldehyde phosphate dehydrogenase, NAD-dependent glyceraldehyde-3-phosphate dehydrogenase, NAD-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase, NAD-dependent phosphorylating glyceraldehyde-3-phosphate dehydrogenase, NAD-G3PDH, NAD-GAPDH, NADH-glyceraldehyde phosphate dehydrogenase, P-37, p-GAPDH, PfGAPDH, phosphoglyceraldehyde dehydrogenase, phosphorylating NAD+-dependent GAPDH, Plasmin receptor, Plasminogen-binding protein, plastidial glyceraldehyde-3-phosphate dehydrogenase, pmGAPDH, PyGapdh, rmGAPDH, Rv1436, somatic GAPD, somatic glyceraldehyde 3-phosphate dehydrogenase, sperm-specific GAPDS, sperm-specific glyceraldehyde 3-phosphate dehydrogenase, sperm-specific glyceraldehyde-3-phosphate dehydrogenase, TaeNAD-GAPDH, TagapC, TDH1, TDH2, TDH3, TLAb, triose phosphate dehydrogenase, UDG, uracil-DNA glycosylase, vGPD

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.12 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)

Cloned

Cloned on EC 1.2.1.12 - glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a 1008 bp DNA fragment from the plr-sdh-gapdh gene is ligated into the vector pGEX-6P-1 for expression of the protein in Escherichia coli BL212DE3 cells
a 1200 bp fragment of the phosphorylating GAPC encoding cDNA is cloned in the antisense orientation under control of the CaMV 35S promoter into the plasmid pBinAR for transformation of Agrobacterium tumefaciens
cloned into the pBluescript vector for expression in Escherichia coli GM-109 cells
-
cloned into the pQE32 vector for expression in Escherichia coli M15 cells
-
determination of complete nucleotide sequence of the coding as well as the noncoding flanking regions of the gene
-
DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli
DNA and amino acid sequence determination of isozyme uracil-DNA glycosylase, UDG
expressed as a histidine fusion protein in Escherichia coli TOP 10 strain
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)-R3 cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli M15 cells
expressed in Escherichia coli strain BL21(DE3)
-
expressed in Escherichia coli strain W3CG and in HeLa cells
-
expressed in Escherichia coli strainW3CG and in HeLa cells
expressed in Escherichia coli TRG8 cells
-
expressed in Escherichia coli XL1-blue cells transformed with pNeis2
-
expressed in HEK-293T cells
-
expressed in MCF-7 cells
-
expressed in prostate cancer cell lines PC-3 and LNCaP
expression in 293Tcells and HepG2.2.15 cells
-
expression in COS-7 cell
-
expression in COS-7 cells
-
expression in Escherichia coli
expression in INS-1 cells
expression in sf21 cell
-
expression of wild-type and mutant enzyme W84F in Escherichia coli
-
FhGAPDH sequence comparisons, functional recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta(DE3)
for expression in Escherichia coli BL21DE
-
Gapdh gene is located on chromosome 14, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), addition of 36 amino acid residues (MGS SHHHHHHSSGLVPRGSHMASMTGGNNMGRGSGF-) at the N-terminus of rPfGapdh
GAPDH gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene cbbG, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene CgGAP or GPD, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, promoter analysis, six stress-response elements were found in the upstream region of the CgGAP gene. Recombinant expression in enzyme-deficient Saccharomyces cerevisiae from plasmid pYX212-CgGAP and functional complementation restoring the wild-type phenotype. The GAP promoter (PCgGAP) is constitutively expressed in Saccharomyces cerevisiae cerevisiae cells grown on glucose, determined via GFP-tagged gene expression analysis
-
gene CgGAP, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, six stress-response elements are found in the upstream region of the CgGAP gene, phylogenetic analysis, funtional complementation of Saccharomyces cerevisiae transformed with CgGAP and restoration to the wild-type when cultured in high-osmolarity medium. Promoter studies in Saccharomyces cerevisiae using the GFP gene as a reporter show that the GAP promoter (PCgGAP) is constitutively expressed in Saccharomyces cerevisiae cells grown on D-glucose
-
gene gap, recombinant expression of His-tagged wild-type and mutant enzymes in Mycobacterium tuberculosis strain H37Ra cell cytosol
gene gapA, complementation of the mutant Escherichia coli strain MG1655DELTAgapA by the Streptococcus mutans gapN gene, EC 1.2.1.9, UniProt ID Q59931, expressed from plasmid pTrcgapN
gene gapA, quantitative real-time PCR enzyme expression analysis, GAPDHS overexpression in melanoma cells (OCM1 cells), phenotype with and without SOX10 knockout, overview
gene gapA, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene gapA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene gapA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) CodonPlus RIPL
gene gapA, recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene gapA, sequence comparisons
gene gapc, targeted expression of plastidial glyceraldehyde-3-phosphate dehydrogenase (GAPCp) in a glyceraldehyde-3-phosphate dehydrogenase double mutant background gapcp1gapcp2
-
gene gapdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha
gene gapdh, recombinant expression in Escherichia coli
-
gene GAPDH1, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, recombinant overexpression in Mortierella alpina using Agrobacterium tumefaciens-mediated transformation
gene GAPDH2, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons, recombinant expression using Agrobacterium tumefaciens-mediated transformation
gene GPD, quantitative RT-PCR enzyme expression analysis, molecular phylogenetic tree using sequences from 26 GAPDH proteins from 12 species of Aspergillus and 8 species of Trichoderma genus
gene Rv1436, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli
genome contains two unlinked genes for D-glyceraldehyde 3-phosphate dehydrogenase: one of them encodes a protein of 37000 Da and the other a protein of 35500 Da
-
into a pGEX-5X-3 and a pGFP-C2 vector
-
into the pET14b vector for expression in Escherichia coli BL21 Star cells
into the pET15b vector for expression in Escherichia coli BL21DE3 cells
-
into the pET28a vector for expression in Escherichia coli BL21DE3 cells, amplificatied in Escherichia coli HB101 cells
-
into the TOPO pET100 expression vector for transformation of Escherichia coli XL1-Blue competent cells
nuclear-encoded, plastid specific enzyme
-
recombinant enzyme expression
recombinant enzyme expression of GST-tagged GAPDH in gapA-deficient Escherichia coli strain DS112
recombinant enzyme expression of GST-tagged tGAPDHS (amino acids 76-408) in gapA-deficient Escherichia coli strain DS112
recombinant enzyme expression of MBP-tagged tGAPDHS (amino acids 106-438) in gapA-deficient Escherichia coli strain DS112
recombinant expression of enzyme gapcp double mutants, gapcp1gapcp2, under the control of photosynthetic (Rubisco small subunit RBCS2B [RBCS]) or heterotrophic (phosphate transporter PHT1.2 [PHT]) cell-specific promoters. Expression of GAPCp1 under the control of RBCS in gapcp1gapcp2 has no significant effect on the metabolite profile or growth in the aerial part (AP). GAPCp1 expression under the control of the PHT promoter clearly affects Arabidopsis thaliana development by increasing the number of lateral roots and having a major effect on AP growth and metabolite profile. Recombinant expression of GFP-tagged isozyme GAPCp1 in Arabidopsis thaliana, quantitative real-time-PCR expression analysis
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Codon PlusR (DE3) RIL
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain HB101
recombinant expression of untagged enzyme in Escherichia coli strain Rosetta 2 (DE3)
recombinant expression of wild-type and mutant enzymes carrying a 20-residue N-terminal tag containing hexa-histidine and a thrombin cleavage sequence in Escherichia coli strain Rosetta (DE3) pLysS
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain HB101
recombinant expression of wild-type and mutant enzymes in HEK-293 cells
recombinant expression of wild-type enzyme in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli strain HB101
recombinant expression of wild-type enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain HB101
sequence comparisons
subcloned into a pET14b vector for expression in Escherichia coli BL21DE3 cells
-
subcloned into the pET15b vector for expression in Escherichia coli cells
the rat cDNA library is cloned in the target vector pTRG, Escherichia coli is used as a reporter strain
-
two genes for glyceraldehyde-3-phosphate dehydrogenase
-