Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.99.1: prostaglandin-endoperoxide synthase

This is an abbreviated version!
For detailed information about prostaglandin-endoperoxide synthase, go to the full flat file.

Word Map on EC 1.14.99.1

Reaction

arachidonate
+
reduced acceptor
 + 2 O2 =
prostaglandin H2
 +
acceptor
 +
H2O

Synonyms

(PG)H synthase, COX, COX-1, COX-2, COX1, Cox2, cyclooxygenase, cyclooxygenase 1, cyclooxygenase 2, cyclooxygenase-1, cyclooxygenase-1b, cyclooxygenase-2, cycloxigenase-2, fatty acid cyclooxygenase, hPGHS-1, hPGHS-2, oPGHS-1, PG G/H synthase 2, PG H synthase, PG synthetase, PG-endoperoxide synthase 2, PG-endoperoxide synthetase, PGH-synthase, PGHS, PGHS isoform-1, PGHS-1, PGHS-2, PHS, PHS-1, PHS-2, prostagladin-H synthase, prostaglandin endoperoxide H synthase, prostaglandin endoperoxide H synthase 1, prostaglandin endoperoxide H synthase 2, prostaglandin endoperoxide H synthase-1, prostaglandin endoperoxide H2 synthase-2, prostaglandin endoperoxide synthase, prostaglandin endoperoxide synthase 2, prostaglandin endoperoxide synthase-1, prostaglandin endoperoxide synthase-2, prostaglandin endoperoxide synthetase, prostaglandin G/H synthase, prostaglandin G/H synthase-2, prostaglandin H synthase, prostaglandin H synthase-1, prostaglandin H synthase-2, prostaglandin H2 synthase, prostaglandin H2 synthase-1, prostaglandin synthase, prostaglandin synthase-2, prostaglandin synthetase, prostaglandin-endoperoxide synthase, prostaglandin-endoperoxide synthase 1, prostaglandin-endoperoxide synthase 2, prostaglandin-H-synthase, prostaglandin-H-synthase 1, prostaglandin-H-synthase 2, PTGS 2, PTGS-1, PTGS-2, PTGS1, PTGS2, putative cyclooxygenase-3, synthase, prostaglandin, tPGHS-1, tPGHS-2

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.99 Miscellaneous
                1.14.99.1 prostaglandin-endoperoxide synthase

Cloned

Cloned on EC 1.14.99.1 - prostaglandin-endoperoxide synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
all mutants are overexpressed in the baculovirus system as detergent-soluble full-length proteins
-
codes for protein of 604 amino acids, 89% identity to human protein
-
COX-1b, expression in COS-7 cells
expressed in colon carcinoma cells COLO320DM
expressed in COS-1 cells
-
expression in Spodoptera frugiperda Sf-9 cells
expression of His-tagged wild-type and mutant COX-2 proteins
expression of isozymes PHS-1 and PHS-2 in CHO-K1 cells
-
expression of mutant enzymes in Sf9 cells
-
for transfection of COS-7 cells
-
functional expression of C-terminally or N-terminally His6-tagged and non-tagged PGHS-2 in Pichia pastoris strain GS115, GS117 and KM71 using native or yeast signal sequences, method evaluation by enzyme activity, overview
into a baculoviral vector for expression in Sf-21 cells
-
into a pVL1392 vector for transformation of Escherichia coli DH5alpha competent cells
into the mammalian expression vector pcDNA3
-
into the pVL1393 vector
-
isozymes 1 and 2
-
PGHS DNA and amiino acid sequence determination and analysis, sequence comparisons
PGHS DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)RP
quantitative real-time RT-PCR enzyme expression analysis
quantitative RT-PCR enzyme expression analysis
stable expression of PHS-1 and PHS-2 in CHO-K1 cells
-