1.14.18.2: CMP-N-acetylneuraminate monooxygenase
This is an abbreviated version!
For detailed information about CMP-N-acetylneuraminate monooxygenase, go to the full flat file.
Reaction
+ 2 ferrocytochrome b5 + + 2 H+ = + 2 ferricytochrome b5 +
Synonyms
CMAH, CMP Neu5Ac hydroxylase, CMP-N-acetylneuraminate hydroxylase, CMP-N-acetylneuraminate,NAD(P)H:oxygen oxidoreductase (hydroxylating), CMP-N-acetylneuraminic acid hydroxylase, CMP-Neu5Ac hydroxylase, CMP-NeuAc hydroxylase, cytidine monophosphate-N-acetylneuraminic acid hydroxylase, cytidine monophospho-N-acetylneuraminic acid hydroxylase, EC 1.14.13.45, oxygenase, cytidine monophosphoacetylneuraminate mono-, pcmah
ECTree
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Natural Substrates Products
Natural Substrates Products on EC 1.14.18.2 - CMP-N-acetylneuraminate monooxygenase
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REACTION DIAGRAM
CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+
CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O
CMP-N-acetylneuraminate + ferrocytochrome b5 + O2 + H+
CMP-N-glycoloylneuraminate + ferricytochrome b5 + H2O
CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O
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CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+
CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O
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?
CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+
CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O
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?
CMP-N-acetylneuraminate + 2 ferrocytochrome b5 + O2 + 2 H+
CMP-N-glycoloylneuraminate + 2 ferricytochrome b5 + H2O
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?
CMP-N-glycoloylneuraminate + ferricytochrome b5 + H2O
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the enzyme determines the sugar bound to the red cell by converting NeuAc to NeuGc. Thus, mutations in CMAH may cause the A and B blood types, two major serotypes. Blood type A is dominant to type B. A rare type AB may also be allelic and is suspected to be recessive to A and dominant to B. Cat blood type antigens are defined: N-glycolylneuraminic acid, NeuGc, is associated with type A and N-acetylneuraminic acid, NeuAc, with type B, overview
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CMP-N-acetylneuraminate + ferrocytochrome b5 + O2 + H+
CMP-N-glycoloylneuraminate + ferricytochrome b5 + H2O
C1K3L2
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?
CMP-N-acetylneuraminate + ferrocytochrome b5 + O2 + H+
CMP-N-glycoloylneuraminate + ferricytochrome b5 + H2O
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ir
CMP-N-glycoloylneuraminate + NAD+ + H2O
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?
CMP-N-acetylneuraminate + NADH + O2
CMP-N-glycoloylneuraminate + NAD+ + H2O
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CMP-N-acetylneuraminate + NADH + O2
CMP-N-glycoloylneuraminate + NAD+ + H2O
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CMP-N-acetylneuraminate + NADH + O2
CMP-N-glycoloylneuraminate + NAD+ + H2O
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binding of CMP-N-acetylneuraminate to CMP-N-acetylneuraminate hydroxylase changes conformation of the enzyme so as to construct a recognition site for cytochrome b5, followed by the formation of a ternary complex through this domain. Then the transport of electrons from NAD(P)H to the enzyme through cytochrome b5 takes place, CMP-N-acetylneuraminate is converted to CMP-N-glycoloylneuraminic acid and finally the ternary complex dissociates into its components to release CMP-N-glycoloylneuraminic acid
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CMP-N-acetylneuraminate + NADH + O2
CMP-N-glycoloylneuraminate + NAD+ + H2O
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the enzyme is the key for regulation of the overall velocity of CMP-NeuAc hydroxylation and consequently for the expression of N-glycoloylneuraminic acid glycoconjugates
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CMP-N-acetylneuraminate + NADH + O2
CMP-N-glycoloylneuraminate + NAD+ + H2O
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a regulation of CMP-N-acetylneuraminate hydroxylation and thus the ratio of glycoconjugate-bound N-acetylneuraminate and N-acetylglycoloylneuraminate might occur by varying the amount of hydroxylase protein within the cell, possibly by controlling the expression of the hydroxylase gene
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CMP-N-acetylneuraminate + NADH + O2
CMP-N-glycoloylneuraminate + NAD+ + H2O
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the enzyme plays a decisive role in governing the relative amounts of N-acetylneuraminate and N-acetylglycolylneuraminate occuring in the glycoconjugates of a tissue
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CMP-N-acetylneuraminate + NADH + O2
CMP-N-glycoloylneuraminate + NAD+ + H2O
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key enzyme for the expression of N-glycoloylneuraminic acid
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CMP-N-acetylneuraminate + NADH + O2
CMP-N-glycoloylneuraminate + NAD+ + H2O
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CMP-N-acetylneuraminate + NADH + O2
CMP-N-glycoloylneuraminate + NAD+ + H2O
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the biosynthesis of the sialic acid N-glycolylneuraminic acid occurs by the action of cytidine monophosphate-N-acetylneuraminate hydroxylase. Incorporation of N-glycoloylneuraminic acid into glycoconjugates is generally controlled by the amount of hydroxylase protein expressed in a tissue
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