1.14.18.2: CMP-N-acetylneuraminate monooxygenase
This is an abbreviated version!
For detailed information about CMP-N-acetylneuraminate monooxygenase, go to the full flat file.
Reaction
+ 2 ferrocytochrome b5 + + 2 H+ = + 2 ferricytochrome b5 +
Synonyms
CMAH, CMP Neu5Ac hydroxylase, CMP-N-acetylneuraminate hydroxylase, CMP-N-acetylneuraminate,NAD(P)H:oxygen oxidoreductase (hydroxylating), CMP-N-acetylneuraminic acid hydroxylase, CMP-Neu5Ac hydroxylase, CMP-NeuAc hydroxylase, cytidine monophosphate-N-acetylneuraminic acid hydroxylase, cytidine monophospho-N-acetylneuraminic acid hydroxylase, EC 1.14.13.45, oxygenase, cytidine monophosphoacetylneuraminate mono-, pcmah
ECTree
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Cofactor
Cofactor on EC 1.14.18.2 - CMP-N-acetylneuraminate monooxygenase
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ferrocytochrome b5
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evidence for the formation of a ternary complex of hydroxylase, CMP-Neu5Ac and cytochrome b5
ferrocytochrome b5
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binding of CMP-N-acetylneuraminate to CMP-N-acetylneuraminate hydroxylase changes conformation of the enzyme so as to construct a recognition site for cytochrome b5, followed by the formation of a ternary complex through this domain. Then the transport of electrons from NAD(P)H to the enzyme through cytochrome b5 takes place, CMP-N-acetylneuraminate is converted to CMP-N-glycoloylneuraminic acid and finally the ternary complex dissociates into its components to release CMP-N-glycoloylneuraminic acid
ferrocytochrome b5
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the electron carrier cytochrome b5 is essential for activity
ferrocytochrome b5
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pure hydrophilic cytochrome b5 interacts more effectively with the hydroxylase than isolated amphiphytic cytochrome b5
ferrocytochrome b5
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pure hydrophilic cytochrome b5 interacts more effectively with the hydroxylase than isolated amphiphytic cytochrome b5
ferrocytochrome b5
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2 electrons are donated by either NADH or NADPH are transported via cytochrome b5 in the CMP-NeuAc hydroxylation system of mouse liver cytosol
NADH
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most effective cofactor, optimal activity at 0.4 mM NADH, higher concentrations are slightly inhibitory